Leukocyte Differential Count Of Long·Talled Macaques (Macacafascicularis)Experimentally Infected With Poliovirus

Stefani Ariesa Wara Hapsari (2017) Leukocyte Differential Count Of Long·Talled Macaques (Macacafascicularis)Experimentally Infected With Poliovirus. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Official URL: http://www.lib.unair.ac.id

Abstract

This research intended to know the effect of injected poliovirus against long-tailed monkeys' leukocyte differential countand whether there is any significant difference in the impact between OPV2 vaccine and WHO Reference poliovirus. This research was led at the Biosafety Level 3 (BSL 3) and Biosafety Level 2 (BSL 2) Laboratories of Avian Influenza Research Center (AIRC) at Universitas Airlangga. The materials utilized as a part of this research are particular pathogenic free (SPF) male or female long-tailed monkeys (Macaca fascicularis) with age around 2-3 years, Oral Poliovirus Vaccine 2 (OPV 2), WHO Reference poliovirus, ketamine and xylazine for anesthesia. The 28 unisex monkeys will be adjusted for 4 to 5 days in BSL 3 research center before the treatment. They were haphazardly divided into two sorts of treatment, OPV2 vaccine and WHO poliovirus, with 14 replications each. The monkeys will be injected with (OPV2 vaccine and WHO Reference poliovirus) and observed for 21 days. The macaques recieved 0.1 ml of poliovirus formulation. Their blood was gathered for pre-treatment and post-treatment hematologic information by means of femoral vein. Both treatment brought in increasing of leukocyte, lymphocyte but not significantly difference (p>O.OOS) whereas monocyte and granulocyte count change significantly difference (p<O,005) between pretreatment and post-treatment datas, but no significant difference revealed between the overall of OPV2 and WHO Reference attenuated poliovirus. The increase of leukocyte differential count is still in normal range

Item Type: Thesis (Skripsi)
Additional Information: KKC KK KH 189-17 Hap l
Uncontrolled Keywords: Leukocyte Differential Count, Lymphocyte, Monocyte, Granulocyte Poliovirus, Macacafascicularis
Subjects: S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF925 Veterinary physical medicine
Divisions: 06. Fakultas Kedokteran Hewan
Creators:
CreatorsNIM
Stefani Ariesa Wara HapsariNIM061311133244
Contributors:
ContributionNameNIDN / NIDK
Thesis advisorChairul Anwar Nidom, -NIDN0008035803
Thesis advisorSetiawan Koesdarto, -NIDN0028095203
Depositing User: Mrs. Djuwarnik Djuwey
Date Deposited: 04 May 2021 05:02
Last Modified: 04 May 2021 05:02
URI: http://repository.unair.ac.id/id/eprint/106636
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