Yudha Kurniawan (2021) Epitope Prediction Of B Cell Gene Encoding F Protein Of Newcastle Disease (Nd) Virus From Pigeon Isolate (Columbia Livia). Skripsi thesis, UNIVERSITAS AIRLANGGA.
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Abstract
Newcastle Disease is a highly contagious disease infecting avian animals caused by the ND virus that was previously synonymous with Avian Paramyxovirus type 1 (APMV-1). Even vaccination program management implemented, Indonesia is still an endemic area for ND. B cell epitope is a sequence of peptides (amino acids) found explicitly on specific antigens' surface, which binds to antibodies. Therefore, epitope characterization is very crucial for the development of vaccine making. There were 20 pigeon samples (Columba livia) collected from bird markets in East Java through the necropsy procedure. Three of them were positively infected by Newcastle Disease. Samples were collected in embryonated chicken eggs and identified by the HA test, confirmed by the HI test. PCR tests were performed on the positive samples using forward and reverse primers with a target of 329 bp. The sequencing result was then translated into an amino acid sequence via BioEdit software version 8.0. Prediction of immunogenic epitopes in genes protein F coding was performed using the online Epitope Prediction software Tools / IEDB with the Bepipred Linear Epitope Prediction. Epitope from sample ND / BG1 / 2019 more likely to be candidates for immunogenic epitopes based on predictions epitope B cell, which has the highest log score of 36.716.
| Item Type: | Thesis (Skripsi) | |||||||||
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| Additional Information: | KKC KK KH.97-21 Kur e | |||||||||
| Uncontrolled Keywords: | Newcastle Disease, F Protein, Amino Acid, B Cell Epitope. | |||||||||
| Subjects: | S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF780.2-780.7 Veterinary microbiology, bacteriology, virology, mycology S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF925 Veterinary physical medicine |
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| Divisions: | 06. Fakultas Kedokteran Hewan | |||||||||
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| Depositing User: | shiefti dyah alyusi | |||||||||
| Date Deposited: | 29 Jun 2021 08:04 | |||||||||
| Last Modified: | 29 Jun 2021 08:04 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/108312 | |||||||||
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