Muhammad Miftahussurur, - and Dalla Doohan, - and Ari Fahrial Syam, - and Iswan Abbas Nusi, - and Langgeng Agung Waskito, - and Kartika Afrida Fauzia, - and Yudith Annisa Ayu Rezkitha, - and Astri Dewayani, - and Reny I'tishom, - and Hasan Maulahela, - and Tomohisa Uchida, - and Yoshio Yamaoka, - (2020) The validation of the Helicobacter pylori CagA typing by immunohistochemistry: nationwide application in Indonesia. Acta Histochemica, 122 (6). pp. 1-7. ISSN 0065-1281

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Abstract

We aimed to validate 2 types of antibodies, anti-CagA antibody and anti-East Asian CagA specific antibody (α-EAS antibody) for the determination of CagA status in Indonesia. We also confirmed the performance of α-EAS antibody for the detection of East Asian-type CagA H. pylori. Immunohistochemistry was performed using anti-CagA antibody and α-EAS antibody on gastric biopsy specimens from a total of 967 Indonesian patients. Diagnostic values of immunohistochemistry were evaluated with PCR-based sequencing as gold standard. Anti-CagA antibody had high sensitivity, specificity, and accuracy (87.0 %, 100 %, and 98.8 %, respectively) for determining CagA status. The α-EAS antibody was not suitable for the purpose of CagA status determination, as it had a low sensitivity (23.9 %). High specificity (97.6 %) but low sensitivity (41.2 %) and accuracy (66.3 %) was observed in α-EAS antibody to detect East Asian-type CagA. Patients with positive result of immunohistochemistry using anti-CagA antibody had significantly higher monocyte infiltration score in antrum (P < 0.001) and corpus (P = 0.009). In conclusion, the anti-CagA antibody is still suitable to be used in Indonesia for determining the CagA status, whilst the α-EAS antibody was not appropriate to discriminate between East Asian-type and non-East Asian-type CagA in Indonesia.

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