Muchammad Yunus, - and Endang Suprihati, - and Agus Wijaya, - (2020) The Endogenous Development of Eimeria tenella in Chickens Injected Subcutaneously with Oocysts Protein as Initially Study of Development of Cecal Coccidiosis Killed Vaccine. Journal of Physics : Conference Series, 1665. pp. 1-5. ISSN 1742-6588

	Text (Prosiding) The Endogenous Development of Eimeria tenella in Chickens Injected Subcutaneously with Oocysts Protein as Initially Study of Development of Cecal Coccidiosis Killed Vaccine.pdf Download (1MB)
	Text (Peer Review) The Endogenous Development of Eimeria tenella in Chickens Injected Subcutaneously with Oocysts Protein as Initially Study of Development of Cecal Coccidiosis Killed Vaccine_Validasi.pdf Download (2MB)
	Text (Turn It In) The Endogenous Development of Eimeria tenella in Chickens Injected Subcutaneously with Oocysts Protein as Initially Study of Development of Cecal Coccidiosis Killed Vaccine (Turn It In).pdf Download (1MB)
	Text (Bukti Sertifikat ISSMART 2020) Bukti_Sertifikat_ISSMART_2020.pdf Download (846kB)
	Text (Form Penilaian Karil Bidang Ilmu) Form_Penilaian_Karil_Bid_Ilmu.pdf Download (213kB)

Abstract

The study was carried out to observe the endogenous development of E. tenella histomorphologically in chickens subcutaneously injected with E. tenella oocyst protein. One day old of twenty-four broiler chickens were divided into 2 groups, each group containing twelve chicks. Group 1 was as control group injected subcutaneously on the neck with two doses: the first dose was administered at age 4th day with Freund's Complete Adjuvant (FCA) emulsified in PBS and administered booster dose was injected at 18th day of age with Freund's Incomplete Adjuvant (FICA) emulsified in PBS. Group 2 was injected subcutaneously on the neck with two doses: first dose at 4th day of age with 50 µg E. tenella oocyst protein emulsified in FCA and booster dose was given at 18th day of age with 50 µg oocyst protein emulsified in FICA. After 14 days of booster, the both groups were challenged orally 1 x 104 of virulent E. tenella. The assessment of endogenous development of E. tenella in chickens evaluated histomorphologically of cecum and oocyst production examination. Injected E. tenella oocyst protein chickens were challenged at 32nd day of age, demonstrated that parasite endogenous development in intestine histomorphologically appeared decreased in proliferation and suppressing oocyst production rate around 68% compared with un-injected chicken. Impaired development of endogenous parasites occurs due to protective immunity resulting from exposure to antigens so that the ability to reproduce and multiply the parasites decreases. The study results demonstrated that relatively sufficient protection against coccidia by use the E. tenella oocyst protein as material of cecal coccidiosis killed vaccine in broiler after challenge. E. tenella oocysts protein can generate protective immunity against homologous challenge through reduction of proliferation parasite and the presence of parasites disabilities. Keywords: oocyst protein, E. tenella, endogenous development

Actions (login required)

View Item