Misgiati (2021) Aktivitas Senyawa Terpenoid Jamur Dewa (Agaricus Blazei Murill) Sebagai Antikanker Sel Mcf-7 Dengan Mekanisme Kerja Induksi Apoptosis. Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The extract of jamur dewa has previously been reported to have anticancer activity in vitro on Hela cells IC50 194.4 μg/ ml, leukemia cells U937, MOLT4, HL60, and K562 IC50 2.7; 9.4; 13.0; and 16.0 g/ml, lung cells IC50 200 g/ml. This study aims to identify the active compound n-hexane of the jamur dewa. Materials and Methods: Isolation of the active compound from the n-hexane extract of jamur dewa was carried out by chromatographic method, and the chemical structure of the isolated compound was determined based on NMR and MS spectra data. MCF-7 cell anticancer assay was determined by the MTT method. The apoptosis induction mechanism is carried out by fluocytometry. Results: A terpenoid compound was isolated from the jamur dewa and identified as ergosterol ([(3)-Ergosta-5,7,22-trien-3-ol]). MCF-7 cell anticancer activity test showed that these compounds could inhibit MCF-7 cells with an IC50 value of 43.12 μg / mL. The mechanism of action test showed that it can induce apoptosis and cell cycle inhibition.

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