Examining Caries Risk With the Caracteristic of HLA-DRB1 Gene

Pratiwi Soesilawati, - and Harianto Notopuro, - and Muhammad Alwino Bayu Firdauzy, - and Inara Putri Prahandita, - (2020) Examining Caries Risk With the Caracteristic of HLA-DRB1 Gene. Malaysian Journal of Medicine and Health Sceinces, 16. pp. 67-70. ISSN 16758544

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Abstract

Introduction: HLA-DRB1 alleles were derived from MHC class II molecules. These alleles encoded sIgA secretion which contribute as a barrier to S. mutans colonization. HLA-DRB1 was known as genes with high mutations causing differences in peptide bond, thus affecting the progression and vulnerability to a disease. The purpose of this study was to determine the effect of mutations in HLA-DRB1 alleles in different dental caries risk. Methods: In this study, we extracted the genomic DNA from whole blood samples of 30 patients with low level dental caries (indeks def-t < 2) as control group and high level dental caries (deft > 3) as case group. HLA-DRB1 varians were studied through genomic DNA isolation for PCR-RFLP. RFLP is analyzed through BseRI, BsaJI, RsaI, and Sau961 restriction enzymes was used in this assay. Results: The PCR-RFLP typing method was evaluated on 60 genomic DNA samples, result found that all samples were divided into 5 groups of variants, two variants in the control group and three variants in the case group. Conclusion: PCR-RFLP was shown to be a sensitive method for the detection of mutation in HLA-DRB1 alleles caused a dental caries level differences. HLA-DRB mutations caused changes in signal transduction and therefore contributes to imunogenetik pathway of dental caries.

Item Type: Article
Subjects: R Medicine
R Medicine > RK Dentistry
Divisions: 02. Fakultas Kedokteran Gigi > Oral Biology
Creators:
CreatorsNIM
Pratiwi Soesilawati, -NIDN0022116903
Harianto Notopuro, -NIDN0013124901
Muhammad Alwino Bayu Firdauzy, --
Inara Putri Prahandita, --
Depositing User: Rudy Febiyanto
Date Deposited: 10 Oct 2021 04:51
Last Modified: 08 Aug 2023 11:40
URI: http://repository.unair.ac.id/id/eprint/110915
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