Sisca Meida Wati, - and Istiati, - and Pratiwi Soesilawati, - (2014) Characterization of lactoferrin in gingival crevicular fluid of chronic periodontitis patient. Dental Journal (Majalah Kedokteran Gigi), 47 (3). pp. 141-145. ISSN 1978-3728

	Text (ARTIKEL) Characterization of lactoferrin in gingival crevicular.pdf Download (260kB)
	Text (SIMILARITY) 21. Characterization of lactoferrin in gingival crevicular fluid of chronic periodontitis patient.pdf Download (1MB)
	Text (VALIDASI) 21. Pratiwi (Characterization of lacyo ) 21.pdf Download (899kB)
	Text (VALIDASI2) 27.Dr.Pratiwi (Characterization of Lactoferrin).pdf Download (781kB)

Abstract

Background: Human periodontal diseases are inflammatory disorders as the result of complex interactions between periodontopathogens and the host’s immune response. Periodontitis results in tooth loss and can even lead to systemic diseases if not treated. Gingival crevicular fluid (GCF) reflects the condition of the gingiva and contains proteins transuded from serum or cells at inflamated sites. Polymorphonuclear leukocyte (PMNs) infiltration can be seen in each stage of periodontitis. Lactoferrin is one of the PMN specific granules and could be a useful marker of PMN activity. Purpose: The aim of this study was to determine the band intensity of lactoferrin used as periodontitis biomarker. Methods: Gingival crevicular fluid (GCF) samples were collected using paper point no.30 from 40 subjects, 30 periodontitis patients that devide according to the severity (10 mild periodontitis, 10 moderate periodontitis, and 10 severe periodontitis) and 10 healthy controls, ranging in ages from 20 to 35 years. GCF lactoferrin was analyzed by Western blot and measured the band intensity by quantity one software (bio-rad). Results: The periodontitis sites exhibited significantly greater band intensity of lactoferrin than healthy sites. The band intensity of lactoferrin was positively correlated with the severity of periodontitis (α = 0.05). Conclusion: The study showed that the intensity of the lactoferrin protein bands could be used as biomarkers of periodontitis.

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