Wiwiek Tyasningsih, - and Y. A. Yurianti, - and Jola Rahmahani, - and Boedi Setiawan, - and Nenny Harijani, - and Budiarto, - and Mustofa Helmi Effendi, - and Salamah, - and A.M. Witaningrum, - (2020) Antimicrobial Resistance Profile of Escherichia Coli Bacteria Collected from Cloaca Swab of Broiler Chicken at Surabaya Traditional Market, Indonesia. Poll Res, 40 (1). pp. 317-321. ISSN 0257-8050

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Abstract

The aim of this research is to identify antimicrobial resistance’s profile of Escherichia coli from cloaca swab of broiler chicken at the Surabaya traditional market for antibiotics: aztreonam, gentamicin, chloramphenicol, tetracycline, and ciprofloxacin. This research is using 60 broilers chicken sample, with cloaca swab method. The samples are isolated and identified with several procedures, Mac Conkey Agar (MCA), Eosin Methylene Blue Agar (EMBA), Gram staining, indole test, Methyl Red-Voges Proskauer (MR-VP), citrate and Triple Sugar Iron Agar (TSIA). The aim of isolation and identification is finding 60 positive isolate of Escherichia coli. Antibiotic sensitivity test using Kirby-Bauer (Disc Diffusion Method) based on Clinical and Laboratory Standard Institute 2018. Escherichia coli sensitivity test proved that resistant bacteria for antibiotic ciprofloxacin is 67%, tetracycline is 65%, gentamicin is 37%, chloramphenicol is 23%, and aztreonam is 3%. KEY WORDS : Antimicrobial, Resistance, Escherichia Coli

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