Jola Rahmahani, - and Suwarno, - and Sri Susila Andayani, - and Kusnoto, - (2009) Wild Type Rabies Virus Glycoprotein to Make Monoclonal Antibody for Early Detection with DAS-ELISA. Proceedings of the International Conference on Animal Health and Human Safety. ISSN 978-967-344-122-8

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Abstract

The objective of this study was to search for wild type rabies virus glycoprotein G protein)for the production of monoclonal antibody for early detection with double antibody sandwich (OAS)-ELISA. This study was divided into several stages, including isolation and determination of type rabies virus with PCR, protein analysis with SOS-PAGE, immunogenicity and antigenicity tests, cloning with limiting dilution technique, purification of immunoglobulin, monoclonal antibody labeling, sensitivity and specificity test of monoclonal antibody used saliva and brain of mice with rabies virus infection. The result showed that the molecular weight ofthe G protein was 62-67 kDa. The result of cloning and recloning rabies virus showed that the virus was wild type (clone Jra 1- ra 8). Sensitivity values of monoclonal antibody used with OAS-ELISA from mice's brain was 95.23% and from mice's saliva was 97.62%. The specificity value of monoclonal antibody from mice's brain was 100% and from mice's saliva was 91.67%.

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