Amelia Hendriana Wijayanti, - and Fedik Abdul Rantam, - and Jola Rahmahani, - (2016) Phylogenetic Analysis and Prediction of Epitope Immunogen Gene Encoding Fusion Protein Newcastle Disease Virus of Blitar Isolate. International Seminar on Molecular Biology in Veterinary Medicine. pp. 65-67. ISSN 978-602-7043-81-7

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Abstract

The aim of this research are to analyze nucleotides sequence, phylogenetic prediction and structure epitope of F protein from Blitar isolate ND virus. Samples of lungs, proventiculus, and feces were collected from chicken infected ND virus in Blitar. Samples were isolated in embryonated eggs and identified by HA test, HI Test, and PCR to confirme of ND virus. The results from PCR were sequenced then analysed used GENETYX program ver. 10. Epitope of F Protein was analyzed using free access online software IEDB program. DNA fragment has been obtained 699 bp Lengths. Base on phylogenetic analysis showed that Blitar ND samples were part of genotype VII, that was genotypically different if compare with isolate starin GenBank. Thus, it could be concluded that epitope prediction of the F protein of ND virus were isolated from Blitar samples has a chance as immunogen candidate, which can be developed as seed vaccine Key words : Newcastle disease, Fusion protein, Blitar

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