Naimah Putri, - and Rahaju Ernawati, - and Jola Rahmahani, - (2021) Application of Spa-Gene as a Molecular Epidemiological Marker in Cases of Methicillin-Resistant Staphylococcus aureus Originating from Dairy Cow's Milk in Surabaya. Jurnal Kedokteran Hewan, 15 (2). pp. 65-70. ISSN 1978-225X(Print), 2502-5600(Electronic)

	Text (Journal) BUKTI APPLICATION OF SPA-GENE AS A MOLECULAR EPIDEMIOLOGICAL MARKER IN CASES OF Methicillin-Resistant Staphylococcus aureus ORIGINATING FROM DAIRY COW'S MILK IN SURABAYA.pdf Download (847kB)
	Text (Peer Review) VALIDASI APPLICATION OF SPA-GENE AS A MOLECULAR EPIDEMIOLOGICAL MARKER IN CASES OF Methicillin-Resistant Staphylococcus aureus ORIGINATING FROM DAIRY COW'S MILK IN SURABAYA.pdf Download (2MB)
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Abstract

The aim of this study was to isolate and identify the strain of methicillin-resistant Staphylococcus aureus (MRSA) from cow’s milk in Surabaya and to determine the fragment Spa-Gene in MRSA strains. There were 50 samples of cow’s milk obtained from four dairy farms. From 50 tested samples, 19 samples (38%) were positive for Staphylococcus aureus. Antibiotic sensitivity test using oxacillin and erythromycin showed that 6 samples were resistant to the antibiotic oxacillin and 6 samples were resistant to erythromycin. MRSA confirmation tests that were conducted on 6 samples revealed 5 positive results for the MRSA strain. Electrophoresis of Polymerase Chain Reaction (PCR) product showed that 4 out of 5 samples were positive for the presence of the Spa-Gene fragment. The research results showed that there were 3 models of Spa-Gene fragments; the first had a length of 90 bp and 140 bp, the second had a length of 140 bp, and third had a length of 90 bp. This study revealed the nature of Spa-Gene polymorphism of MRSA strains isolated from milk samples. It was concluded that the Spa-Gene can be used as a molecular epidemiological marker of the MRSA strain. Key words: methicillin-resistant Staphylococcus aureus (MRSA), Spa-Gene, polymorphism, Staphylococcus aureus

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