Iwan Sahrial Hamid, - (2021) Antiangiogenic Effect of Ficus Carica Linn Leaf Extract on Total Blood Vessels, Matrix Metalloproteinase-9 Expression and Macrophages in the Chik Embryo Chorioallantoic Membrane. Journal Systematic Reviews in Pharmacy, 12 (1). pp. 1282-1286. ISSN 0975-8453

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Abstract

Background and Aim: Inhibiting angiogenesis is suppose to be more effective in preventing potential cell metastases. Antiangiogenic substances inhibit the supply of nutrients and oxygen to cancer cells so that the development of protooncogens can be inhibited. This study aimed to evaluate the effect of Ficus carica Linn leaf extract as antiangiogenic on total blood vessels, matrix metalloproteinase-9 (MMP-9) expression and total macrophages on chorioalantoic membranes of chicken embryos. Materials and Methods: A total of 25 chicken embryos were randomly assigned to 5 treatment groups, i.e. (C-) induced by Tris-HCl and 2% DMSO solvent; (C +) induced 60 ng of bFGF and Tris-HCl solvent; (T1) induced 60 ng of bFGF and 20 µg of celecoxib; (T2) induced 90 ng of bFGF and 90 µg of F. carica L. extract in 2% DMSO; (T3) was induced by 60 ng of bFGF and 110 µg of F. carica L. leaf extract in 2% DMSO. The total blood vessels observed macroscopically were counted on the main blood vessel branches around the paper disc. Total macrophages were observed on HE staining and MMP-9 expression on IHC staining. Data were analyzed by ANOVA test followed by Tukey's test (p <0.05). Results: The results show that T3 was significant (p<0.05) compared to C+ and T2 for all observed parameters. Meanwhile, T3 was not significant (p>0.05) compared to C- and T1 for all observed parameters. Conclusion: It can be concluded that F. carica L. leaf extract at a dose of 110 µg in DMSO 2% can significantly reduce blood vessel total, MMP-9 expression and total macrophages in chicken embryos induced by bFGF. On the other hand, there was no significant difference between F. carica L. leaf extract at a dose of 110 µg in DMSO 2% with celecoxib treatment, which means that it gave the same effectiveness in reducing the effect of bFGF Key words: Antiangiogenic, Ficus carica Linn, macrophage, matrix metalloproteinase

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