Wiwiek Tyasningsih, - and Didik H, - and Fedik Abdul Rantam, - and Aulanni’am, - (2015) The Characterization of Gene Encoding Surface Protein-31(BCSP-31) Brucella abortus Field Isolate By PRC Technique. International Journal of Advance Research In Science And Engineering, 4 (1). pp. 223-227. ISSN 2319-8354

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Abstract

Brucellosis is a major bacterial zoonosis of global importance. The causative organisms are Gramnegative facultative intracellular pathogens, that may affect a range of different mammals including man, cattle, sheep, goats, swine, rodents and marine mammals. The disease primarily affects the reproductive system with concomitant loss in productivity of animals affected. Human infection is characteristically recurrent febrile as known undulant fever. The development of an effective subunit vaccine against brucellosis is a research interest. The protein antigenic of cell envelope Brucella sp. have been extensively characterized as potential immunogenic and protective antigens. The aim of the study was to characterize the gene encoding surface protein-31Brucella abortus (BCSP-31) field isolate as the basic for the molecular diagnosis of the disease Brucellosis in animals. The method used in this study is the isolation and reidentification of Brucella abortus bacteria based on biochemical tests and genomics with Polymerase Chain Reaction (PCR); the result of the PCR was then performed and analyzed sequensing by Blast homology. The data were derived from field isolates of Brucella abortus. The results showed that the gene Surface Protein-31 Brucella abortus (BCSP31)field isolat had a size of 224 bp, and the homology analysis on Gene Bank the field isolate Brucella abortusshowed that the homology is 97% with the others strainsB. abortus in the world including B. abortus S19. Key words: Gen Surface Protein-31(BCSP-31), B. abortus field isolate, PCR Sequence

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