Jefry Wahyudi Safril, - and Ni Putu Mira Sumarta, - and Gatot Baydowi, - and Coen Pramono Danoediningrat, - (2018) Effect of different concentration of alpha tocopherol supplementation on human bone marrow mesenchymal stem cell viability (in vitro study). Asian Jr. of Microbiol. Biotech. Env. Sc., 20. S121-S123. ISSN 0972-3005

	Text (ARTIKEL) 4 EFFECT OF DIFFERENT CONCENTRATION.pdf Download (165kB)
	Text (SIMILARITY) 4_ EFFECT OF DIFFERENT CONCENTRATION OF ALPHA TOCOPHEROL SUPPLEMENTATION ON HUMAN BONE MARROW MESENCHYMAL STEM CELL VIABILITY (IN VITRO STUDY).pdf Download (771kB)
	Text (VALIDASI) 4 EFFECT OF DIFFERENT CONCENTRATION-validasi.pdf Download (3MB)
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Abstract

Bone defect can be reconstructed with autogenous bone graft and considered as an ideal for bone graft material. The process of bone regeneration that occurs because of the proliferation and differentiation of human bone mesenchymal stem cell marrow. Alpha tocopherol is a co-factor that can help osteogenesis as micronutrient supplementation. Biocompability of this supplement still needs to be proven. This study is to determine the effect of alpha tocopherol supplementation on human bone marrow mesenchymal stem cell viablity. Experimental studies performed in vitro on human bone marrow mesenchymal stem cell. This study was divided into 2 main groups: alpha tocopherol concentrations group (25 μM/mL, 50 μM/mL, 75μM/mL) and control group. Each group is analyzed with MTT assay observed living cells. Alpha tocopherol with 25 μM/mL concentration obtained high results (107,86%) than other samples (p>0,05 in all subjects observed). Optimum concentration of Alpha tocopherol is 25 μM/ml.

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