Ni Putu Mira Sumarta, - and David Buntoro Kamadjaja, - and Nike Hendrijantini, - and Coen Pramono Danudiningrat, - and Fedik Abdul Rantam, - (2021) Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine. Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 21. pp. 1-8. ISSN 1519-0501

	Text (ARTIKEL) 12 Human Umbilical Cord Mesenchymal.pdf Download (1MB)
	Text (SIMILARITY) 12_ Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine.pdf Download (3MB)
	Text (VALIDASI) 12 Human Umbilical Cord-Validasi.pdf Download (4MB)
	Text (KARIL) 12.pdf Download (1MB)
	Text (TURNITIN) Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine.pdf Download (3MB)
	Text (KORESPONDEN) koresponden Human Umbilical.pdf Download (76kB)

Abstract

Objective: To evaluate the regeneration of mandibular cartilage defect after implantation of human umbilical cord mesenchymal stem cells (hUCMSC) over platelet rich fibrin (PRF) as scaffold. Material and Methods: 20 male Wistar rats were randomly divided into four experimental groups consisting of: a control group featuring untreated mandibular defects (C), experimental groups whose mandibular defects were implanted with hUCMSC (E1), mandibular defects implanted with PRF (E2), mandibular defects implanted with hUCMSC and PRF scaffold (E3). The subjects were sacrificed after six weeks of observation for immunohistochemical examination in order to evaluate the expression of Ki67, Sox9, FGF 18, type 2 collagen, and aggrecan, in addition to histology examination to evaluate chondrocyte number and cartilage thickness. Data was analyzed with univariate analysis (ANOVA). Results: The implantation of hUCMSC and PRF scaffold proved capable of regenerating mandibular cartilage defect through the expression of FGF 18, Sox9, Ki67, chondrosis counts, type 2 collagen, aggrecan, and cartilage thickness. The regeneration were significantly higher in group E3. Conclusion: Human umbilical cord mesenchymal stem cells in platelet rich fibrin scaffold proved capable of regenerating mandibular cartilage defect.

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