Silvina Resti Lestari, .- and Sugiharto, .- and Alfinda Novi Kristanti, .- and Yosephine Sri Wulan Manuhara, .- (2018) Biomass and flavonoid production of Gynura procumbens (Lour.) Merr. axillary shoots culture induced by source and erythrosa-4-phosphate. Scholars Academic Journal of Biosciences (SAJB), 5 (4). pp. 257-263. ISSN 2321-6883 (Online)

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Abstract

Gynura procumbens (Lour.) Merr. is a medicinal plant which was used in Indonesia, Malaysia, Thailand, and another region of South East Asia. These plants contain many compounds which useful as source of medicine like flavonoid. In order to increase biomass and flavonoid production, in vitro axillary shoots culture were initiated from stem node. Axillary shoot were cultured in Murashige and Skoog (MS) medium supplemented with 2 mg/L indol acetic-acid, 4 mg/L benzyl adenine, sucrose (10, 30, 50 g/L), and erythrose 4-phosphate (0, 1, 2.5, 5, 25 µM). Results showed that the highest fresh weight was achieved in medium supplemented with sucrose 50 g/L and erythrose 4-phosphate 5 µM, whereas supplemented of sucrose 30 g/L and erythrose-4-phosphate 5 µM gave the highest dry weight. Increasing supplementation of sucrose will increase cathecine content in all treatment. The highest concentration of quercetin and kaempferol was achieved in medium supplemented with sucrose 30 mg/L and erythrose 4-P 1 µM.

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