Puspa Wardhani, - and Aryati, - and Benediktus Yohan, - and Hidayat Trimarsanto, - and Tri Y. Setianingsih, - and Dwiyanti Puspitasari, - and Muhammad Vitanata Arfijanto, - and Bramantono, - and Suharto, - and R. Tedjo Sasmono, - (2017) Clinical and virological characteristics of dengue in Surabaya, Indonesia. PloS ONE, 12 (6). pp. 1-21. ISSN 19326203

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Abstract

Dengue disease is still a major health problem in Indonesia. Surabaya, the second largest city in the country, is endemic for dengue. We report here on dengue disease in Surabaya, investigating the clinical manifestations, the distribution of dengue virus (DENV) serotypes, and the relationships between clinical manifestations and the genetic characteristics of DENV. A total of 148 patients suspected of having dengue were recruited during February-August 2012. One hundred one (68%) of them were children, and 47 (32%) were adults. Dengue fever (DF) and Dengue hemorrhagic fever (DHF) were equally manifested in all of the patients. We performed DENV serotyping on all of the samples using real-time RT-PCR. Of 148, 79 (53%) samples were detected as DENV positive, with DENV-1 as the predominant serotype (73%), followed by DENV-2 (8%), DENV-4 (8%), and DENV-3 (6%), while 5% were mixed infections. Based on the Envelope gene sequences, we performed phylogenetic analyses of 24 isolates to genotype the DENV circulating in Surabaya in 2012, and the analysis revealed that DENV-1 consisted of Genotypes I and IV, DENV-2 was of the Cosmopolitan genotype, the DENV-3 viruses were of Genotype I, and DENV-4 was detected as Genotype II. We correlated the infecting DENV serotypes with clinical manifestations and laboratory parameters; however, no significant correlations were found. Amino acid analysis of Envelope protein did not find any unique mutations related to disease severity.

Item Type:	Article
Uncontrolled Keywords:	Clinical and virological, dengue
Subjects:	R Medicine > R Medicine (General) R Medicine > RB Pathology > RB37-56.5 Clinical pathology. Laboratory technique
Divisions:	01. Fakultas Kedokteran > Patologi Klinik
Creators:	Creators NIM Puspa Wardhani, - NIDN0025027506 Aryati, - NIDN0015086304 Benediktus Yohan, - UNSPECIFIED Hidayat Trimarsanto, - UNSPECIFIED Tri Y. Setianingsih, - UNSPECIFIED Dwiyanti Puspitasari, - NIDN0016107404 Muhammad Vitanata Arfijanto, - NIDN0015097112 Bramantono, - NIDN8886800016 Suharto, - UNSPECIFIED R. Tedjo Sasmono, - UNSPECIFIED
Depositing User:	arys fk
Date Deposited:	11 Mar 2022 07:00
Last Modified:	11 Mar 2022 07:00
URI:	http://repository.unair.ac.id/id/eprint/113750
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