May Fanny Tanzilia, - and Nelly Zuroidah, - and I Gusti Agung Ayu Eka Putri Sunari, - and Billy Jordan Wrahatnala, - and Faradila Khoirun Nisa, - and Hakim, - and Ali Rohman, - and Puspa Wardhani, - and Dominicus Husada, - and Siti Nadia Tarmizi, - and Aryati, - (2020) Comparative Diagnostic Value of Anti-Dengue IgG, Anti-Dengue IgM of Two Rapid Tests in Dengue Virus Infection. International Journal of Pharmaceutical Research, 12 (4). pp. 1657-1664. ISSN 09752366

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Abstract

Introduction: Diagnosis of Dengue Virus Infection (DVI) is based on combination of clinical manifestation, hematological, serological, and molecular examination. This study aims to compare the diagnostic value of anti-dengue IgG and anti-dengue IgM by two rapid tests with RT-PCR Dengue and ELISA as the gold standards. Methods: This was an observational cross-sectional study. Serum was collected from 80 febrile patients (3-7 day fever), who were suspected Dengue Virus Infection (DVI). Serum was examined by STANDARD Q-SD and SD BIOLINE as rapid tests and RT-PCR and or NS1 Ag Dengue (ELISA) and anti-dengue IgG/IgM ELISA as the gold standards. Results: The most common serotype identification was DENV 3. The positive results of NS1 Ag Dengue were common on day 3 of fever in primary infection and day 4 of fever in secondary infection. The antibody examination was dominated by positive anti-dengue IgM and positive anti-dengue IgG in 27 patients (54%) in secondary infection. Sensitivity anti-dengue IgG STANDARD Q was 68%, and SD BIOLINE was 87%. Specificity anti-dengue IgG STANDARD Q was 80%, and SD BIOLINE was 76.7%. Sensitivity IgM anti-dengue STANDARD Q was 44%, and SD BIOLINE was 48%. Specificity anti-dengue IgM of two rapid tests was 100%. Conclusion: The serological examination with rapid tests is not enough to establish a diagnosis of DVI, so the combination with NS1 Ag Dengue and RT-PCR examination is needed.

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