Yovilianda Maulitiva Untoro, - and Teguh Hari Sucipto, - and Harsasi Setyawati, - and Siti Churrotin, - and Ilham Harlan Amarullah, - and Puspa Wardhani, - and Aryati, - and Shuhai Ueda, - and Soegeng Soegijanto, - (2018) RNA Isolation of Dengue Virus Type 2 With Different Precipitation Solvents: Methanol, Chloroform, And 2-Isopropanol. Jurnal Kimia Riset, 3 (1). pp. 52-57. ISSN 2528-0422

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Abstract

Dengue virus distributed in tropical and subtropical regions in the world. DENV viruses are transmitted between humans primarily by Aedes aegypti and Aedes albopictus mosquitoes and are endemic in most areas in which the vectors occur. Four serotypes of dengue virus are DENV-1, DENV-2, DENV-3 and DENV-4. DENV-2 is comprised of six genotypes. Ethanol precipitation is a commonly used technique for concentrating and de-salting nucleic acids (DNA or RNA) preparations in aqueous solution. RNA isolation by combining Guanidinium thiocyanate and phenol reported has been reported. In this report, we investigated RNA isolation from DENV-2 using QIAamp Mini Kit with 2-Isopropanol, Methanol, Chloroform precipitation solvent. Electrophoregram showed DNA band as the result of RNA isolation with methanol and 2-isopropanol are produced quite well. Dna band of the of RNA isolation with chloroform solvent has the lowest intensity than methanol and 2-isopropanol. This study showed that methanol and 2-isopropanol can used as precipitation solvent for isolating RNA.

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