M H Solim, .- and A N Kristanti and Y S W Manuhara, .- (2017) Influence of explant position on growth of Talinum paniculatum Gaertn. adventitious root in solid medium and enhance production biomass in baloon type bubble bioreactor. In: IOP Conf. Series: Earth and Environmental Science. IOP Conf. Series: Earth and Environmental Science, 58 . IOP Publishing, bogor, Indonesia. ISBN 1755-1307 ; 1755-1315

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Abstract

Abstract. Talinum paniculatum Gaertn. is one of traditional medicinal plant in Indonesia as an aphrodisiac. This plant has various compounds which is accumulated in roots. In vitro culture of this plant can enhance production of adventitious roots. The aim of this research was to know the influence of explants position on growth of T. paniculatum Gaertn. adventitious root in MS solid medium and enhance the production of biomass in balloon type bubble bioreactor. Explants from leaf were cultured at abaxial and adaxial positions in solid MS medium supplemented with IBA 2 mgL-1. Adventitious roots were cultured in bioreactor with various treatments (without IBA, supplemented with IBA 2 mgL-1 and supplemented with IBA 2 mgL+ buffer NaHCO). Result showed that the main growth of abaxial root was higher than adaxial, however, the total of adaxial root branch was higher than abaxial. The highest biomass production of adventitious root cultured was achieved by MS medium supplemented with IBA 2 mgL-13 + buffer NaHCO. This treatment has produced fresh biomass two fold of initial inoculum.

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