Mangestuti Agil and Retno Widyowati, - and NOOR ERMA SUGIJANTO N. (2012) Fraksinasi dan Uji Daya Hambat Bakteri Mycobacterium tuberculosis dari Ekstrak 11-Heksana dan Metanol Umbi Bidara Upas (Merremia mammosa Hall.). Laporan Penelitian. UNIVERSITAS AIRLANGGA, SURABAYA. (Unpublished)

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Abstract

Bidara upas (Merremia mammosa (Lour.) Hallier f.) is known as traditional antiuberculosis plant in Madura. This study was aimed to investigate antibacterial activity of actions of n. hexane and methanol of Bidara upas inhibiting Mycobacterium Tuberculosis growth. These fractions were made by using vacuum liquid chromatography methods with hexane and a mixture of chloroform and methanol as the eluent. A former research showed n.hexane and methanol extracts contained flavonoid and terpenoid components. he antibacterial test for n. hexane, fraction E showed antibacterial activity against ycobacterium tuberculosis H3 7RV A TCC 27294 at the concentration of 400-12,5 J.lg/ml. I MI C has not been detected yet. I antibacterial test for methanol extract, 9 fractions at 500 J.lg/ml concentration was c osen to be analyzed the antibacterial activity. The result showed only one fraction, k own as fraction 4, showed antibacterial activity against both Mycobacterium t berculosis H37Rv 27294 strains. The MIC of fraction 4 is 50 J.lg/ml.

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