David Buntoro Kamadjaja, - and Zefry Zainal Abidin, - and Riska Diana, - and Ikhram Kharis, - and Ni Putu Mira Sumarta, - and Muhammad Subhan Amir, - and Andra Rizqiawan, - and Coen Pramono Danudiningrat, - and Norifumi Nakamura, - (2021) In Vivo Analyses of Osteogenic Activity and Bone Regeneration Capacity of Demineralized Freeze-Dried BovineBoneXenograft:A Potential Candidate for Alveolar Bone Fillers. Hindawi International Journal of Dentistry, 2021. pp. 1-10. ISSN 20906447

	Text (ARTIKEL) 21 In Vivo Analyses of Osteogenic Activity.pdf Download (5MB)
	Text (TURNITIN) 20_The Effect of the Application_turnitin.pdf Download (1MB)
	Text (VALIDASI) 21 In Vivo Analyses of Osteogenesis_validasi.pdf Download (3MB)
	Text (KARIL) 16.pdf Download (1MB)
	Text (TURNITIN 2) turnitin in Vivo.pdf Download (5MB)

Abstract

Background. Deproteinized bovine bone mineral (DBBM) particle is the commonly used bone graft substitute in implant surgery which is mainly osteoconductive and has very slow degradation. Demineralized freeze-dried bovine bone xenograft (DFDBBX) particle is being developed as a novel xenogeneic bone filler. Objectives. +e study aimed to analyze osteogenic activity and boneforming capacity of DFDBBX particles compared to DBBM particles in alveolar bone defects in rabbit mandibles models. Material and Methods. +is study investigated bone defects whether filled with DBBM particles or DFDBBX particles or left unfilled in 30 rabbit mandibles. Specimens were processed for histology, immunohistochemistry, and micro-CT scanning. Statistical difference was set at a p value < 0.05. Results. +e quantitative assessment showed a significantly lower number of osteoclasts and a higher number of osteoblasts in the DFDBBX group compared to the DBBM group in 2 and 4 weeks (p < 0.05). Immunostaining analyses showed significantly higher expression of RUNX2, collagen type I, alkaline phosphatase, and osteocalcin in the DFDBBX group compared to the DBBM group in 2 and 4 weeks. Bone healing score in the DFDBBX group was comparable to the DBBM group. Micro-CT presented no significant difference in the volume percentage of the mineralized tissue in the DBBM and DFDBBX groups in spite of the different healing patterns in both groups. Conclusion. DFDBBX particles induced higher osteoblastic activities than DBBM particles at the early stage of healing. Meanwhile, the capacity of bone formation in DFDBBX particles was comparable with DBBM particles at the later stage of healing. Considering the limitation of this study, the results presented DFDBBX particles as potential bone filler candidates.

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