E. Munadziroh, - and N.N. Tripuspaningsih, - and Soetjipto, - and M. Rubianto, - (2009) Isolation and Identification of Java Ras Amniotic Membrane SLPI Gene. Laporan Penelitian. LEMBAGA PENELITIAN, Surabaya. (Unpublished)
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Abstract
Secretory leukocite protease inhibitor (SLPI) is a protein found in amniotic membrane that involve in tissue repair and oral wound healing process. The oral wound healing responses are impaired in the SLPI suffic ient mice and matrix synthesis and collagen deposition are delayed. Impaired healing states are characterized by excessive proteolysis and often bacterial infection. SLPI is a protein fo und in secretions such as whole saliva, seminal fluid, cervical mucus, as in secretions f rom the nose and bronchi, amniotic fluid and amniotic membrane etc .. The objective of this research is to isolated and cloning the SLPI from human amniotic membrane and determined the expression of SLPI extracted from human amniotic membrane from Java ras. Methods: cDNA isolated from human amniotic membrane was amplified by reverse transcription polymerase chain reaction (RTPCR). After the amplified, fragmen were cloned into the expression pET IO llDl'OPO Vector and transformed to BL-21 Star. The insert position, size and the readingframe were right by PCR .. Result: Through sequence analyses, SLPI cDNA was 495 nucleotide in length with a predicted molecular mass of about 12 kDa (the data was registered in genbank (htpp:llwww.ncbi.nib.gov) with accession number EUI16331j. COllclusion: It is demonstrated that human SLPI are highly conserved in sequence content as compared to the human SLPI f rom gene bank.
| Item Type: | Monograph (Laporan Penelitian) | ||||||||||
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| Additional Information: | KKA KK LP 164 / 10 ISO | ||||||||||
| Uncontrolled Keywords: | Secretory leukocyte protease inhibitor, amniotic membrane, cloning. Recombinant, wound healing | ||||||||||
| Subjects: | R Medicine > RK Dentistry > RK1-715 Dentistry | ||||||||||
| Divisions: | 02. Fakultas Kedokteran Gigi > S1 Kedokteran Gigi | ||||||||||
| Creators: |
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| Depositing User: | Dewi Puspita | ||||||||||
| Date Deposited: | 13 May 2022 00:40 | ||||||||||
| Last Modified: | 13 May 2022 00:40 | ||||||||||
| URI: | http://repository.unair.ac.id/id/eprint/116111 | ||||||||||
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