Retno Widyowati, - and Neny Purwitasari, - and Rice Disi Oktarina, - and Wiwied Ekasari, - and Saarah Khairunnisa, - and Hsin-I. Chang, - (2021) Exploration of several plants from Baung Forest on bone formation cell models. Journal of Basic and Clinical Physiology and Pharmacology, 32 (4). pp. 831-837. ISSN 0792-6855

	Text (FULLTEXT) C-27_artikel.pdf Download (1MB)
	Text (PEER REVIEW) C-27_peer review.pdf Download (974kB)
	Text (SIMILARITY) C-27_similarity.pdf Download (2MB)
	Text (FULLTEXT (RW)) C-03_artikel.pdf Download (1MB)
	Text (Kualitas Karil (RW)) Kualitas Karil C-03.pdf Download (486kB)
	Text (Similarity (RW)) C-03_similarity.pdf Download (2MB)
	Text (Korespondensi (RW)) C-03 korespodensi.pdf Download (718kB)
	Text (Korespondensi REVISI (RW)) Korespondensi C-03 rev.pdf Download (3MB)

Abstract

Objectives Osteoporosis is an ailment described by a skeletal degradation of bone skeletal dominating to increases the chance of fracture. In order to find out the bone formation agents from Baung Forest plants, this research analyzed the effects of 96% ethanol extract of several plants from Baung Forest on antioxidant activity and the effect of osteoblast differentiation-related to the bone formation on the most potent extract. Methods The antioxidant effect and osteoblast differentiation of 96% ethanol extracts were evaluated by measuring DPPH scavenging and alkaline phosphatase in p-nitrophenyl phosphate effects by the enzyme-linked immunosorbent assay (ELISA) reader method, respectively. Results The 96% ethanol extract of Elaeocarpus serratus L. from Baung Forest had the strongest DPPH radical scavenging as anti oxidant (82.17%) and stimulated osteoblast differentiation (116%). Then, this extract had been fractionated based on polarity to become hexane, ethyl acetate, butanol, and aqueous fractions. All the fractions stimulated their alkaline phosphatase (ALP) activity to 138.11 ± 9.72%, 108 ± 5.05%, 148.56 ± 8.47, and 144.58 ± 1.04, respectively. Conclusions The extract and fractions of E. serratus L. can successfully inhibit DPPH radical scavenging value and increase ALP activities as markers of osteoblast functions.

Actions (login required)

View Item