Ryo Uetsuki, - and Koichiro Higashikawa, - and Satoshi Okuda, - and Nao Yamakado, - and Fumi Ishida, - and Andra Rizqiawan, - and Shigehiro Ono, - and Masaaki Takechi, - and Kuniko Mizuta, - and Hideo Shigeishi, - and Nobuyuki Kamata, - and Kei Tobiume, - (2021) The squamous cell carcinoma cell line OM-1 retains both p75-dependent stratified epithelial progenitor potential and cancer stem cell properties. Biochemistry and Biophysics Reports, 26. pp. 1-7. ISSN 2405-5808

	Text (NASKAH) 12.The squamous cell carcinoma.pdf Download (4MB)
	Text (TURNITIN) 10. tiurnitin The squamous cell carcinoma cell line.pdf Download (2MB)
	Text (VALIDASI) Artikel 12 Andra Rizqiawan.pdf Download (3MB)
	Text (KARIL) Karil 19 Prof Indri SCC OM1.pdf Download (134kB)

Abstract

The low-affinity nerve growth factor receptor p75 is a stratified epithelial stem/progenitor marker of human epithelia. We found OM-1, a human squamous cell carcinoma (SCC) cell line, showed distinct cells with p75 cluster, especially located at the center of a growing colony in a monolayer culture. A cell with p75 cluster was surrounded by cytokeratin 14- and cytokeratin 13-expressing cells that settled at the outer margin of the colony. OM-1 cells were also capable of forming tumor spheres in a cell suspension culture, an ability which was attenuated by the inhibition of p75-signaling. Intriguingly, we also found a p75-negative cell population from a growing culture of OM-1 that re-committed to become p75-clustering cells. These results indicated the possibility that SCC with epithelial multi-layering capacity can exploit the p75-dependent stratified epithelial progenitor property for the cancer stemness.

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