Citrus limon Peel Essential Oil–Induced Type IV Hypersensitivity Reaction

Fatma Yasmin Mahdani, - and Adiastuti Endah Parmadiati, - and Diah Savitri Ernawati, - and Hasrul Husain, - and Sekar Alifa Putri Ekaperdana, - and Ulyasari Rachmaningayu, - and Priyo Hadi, - and Hening Tuti Hendarti, - and Meircurius Dwi Condro Surboyo, - (2020) Citrus limon Peel Essential Oil–Induced Type IV Hypersensitivity Reaction. Journal of Experimental Pharmacology, 12 (-). pp. 213-220. ISSN -

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Background: Several studies have shown anti-inflammatory, anti-microbial, antifungal, and antioxidant effects from Citrus limon–peel essential oil (Cl-PEO). Cl-PEO can be developed as topical drugs for oral ulceration because of its potential active components. There have been no studies on the topical application of Cl-PEO inducing type IV hypersensitivity reaction. Purpose: To investigate the potential of Cl-PEO from Batu City to induce type IV hypersensitivity reactions based on clinical changes, lymphocytes, macrophages, IFNγ, andIL10 expression. Methods: This study was adapted from a guinea pig maximization-test method in Indonesia and the guidance of ISO 10,993-10:2010, and conducted on 20 guinea pigs (Cavia cobaya) divided into a control group and a treatment group. The treatment group was given Cl-PEO and the control group CMC-Na. Clinical changes were observed, then tissue specimens taken for hematoxylin–eosin and immunohistochemistry staining. Results: There were no clinical changes after exposure. Lymphocyte and macrophage numbers and IFNγ and IL10 expression increased in the treatment group compared to the control group (p=0). Conclusion: Cl-PEO can induce type IV hypersensitivity reactions in guinea pigs based on cellular and molecular cytokines, but there are no clinical changes after topical application.

Item Type: Article
Uncontrolled Keywords: Citrus limon, essential oil, type IV hypersensitivity, macrophages, lymphocytes, interferon gamma, interleukin 10
Subjects: R Medicine > RK Dentistry
R Medicine > RK Dentistry > RK1-715 Dentistry
Divisions: 02. Fakultas Kedokteran Gigi > Ilmu Penyakit Mulut (Spesialis)
Fatma Yasmin Mahdani, -NIDN0012128802
Adiastuti Endah Parmadiati, -NIDN0017055903
Diah Savitri Ernawati, -NIDN0029046007
Hasrul Husain, --
Sekar Alifa Putri Ekaperdana, --
Ulyasari Rachmaningayu, --
Priyo Hadi, -NIDN9990133190
Hening Tuti Hendarti, -NIDN9990147693
Meircurius Dwi Condro Surboyo, -NIDN0030059010
Depositing User: Muhammad Fadli Rois
Date Deposited: 07 Sep 2022 04:33
Last Modified: 07 Sep 2022 04:33
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