Rinda Dewi Safitri, - and Risi Cicilia, - and Intan Galuh Bintari, - and Intan Permatasari Hermawan, - and Mustofa Helmi Effendi, - and Rahaju Ernawati, - and Jola Rahmahani, - (2017) Detection Of Encoding Gene Extended Spectrum Beta Lactamase On Escherichia Coli Isolated From Broiler Chicken Meat In Traditional Market Surabaya. International Journal Of Development Research, 7 (11). pp. 17354-17357. ISSN 2230-9926

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Abstract

This study aims to isolate, to identify, and to seek out fragments of encoding gene Extended Spectrum β-Lactamase on Escherichia coli isolated from swab surface of broiler chicken meat in a number of traditional markets in Surabaya. The result shows that 31 out of 50 samples positively contain Escherichia coli, shown through EMBA isolation media and identified using indole test. Sensitivity test shows that 100% of the isolates are resistant to Ampicilin, 48.4% are resistant to Cephazoline, 13% are resistant to Ceftazidime, 9.6% are resistant to Cefotaxime, 6.4% are resistant to Ceftriaxone and 87.2% are resistant to Tetracycline. 8 out of 8 (100%) samples of E. coli resistant show the presence of band towards blaTEM gene of 861basepair (bp) Key Words: Extended Spectrum β-Lactamase,Escherichia coli,broiler chicken meat,blaTEM gene,Antimicrobial Resistance.

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