Junaidi Khotib, - (2009) Protective Effect Of Vanadyl Sulphate On The Morphological Change Of Pancreas, Muscle And Adipose , Tissues In Streptozotocin-Induced Diabetic Mice. Laporan Penelitian. LEMBAGA PENELITIAN UNAIR, SURABAYA. (Unpublished)

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Abstract

This present study is designed to investigate the protective effect of vanadyl sulphate on the morpbological change of pancreas, muscle and adipose tissues in streptozotocin-induced diabetic mice. The diabetic mice model was induced twice by intraperitoneally administration of streptozotocin at 100 mglkg bexly weight followed by 50 mglkg at 14 days after the first injection. To observe the development of diabetes mellitus, blood glucose level was measured on day 0, 7, 14 and 21 with portable glucometer. On day 21, vanadyl sulphate suspension at 5,30, or 100 mglkg was given orally once a day for 7 consecutive days. The mice were sacrificed on the day 28. Then, pancreas, muscle, and adipose tissues were dissected for histopathological approaches. We found that the islet langerhans and number of beta pancreatic cells were significantly decreased in the diabetic group. In addition, the diameter of hind paw muscle and glycogen were drastically reduced. Likewise, there was a structural change of adipose ti ssue and a decrease in fatty drop. TIle repeated treannents of vanadyl sulphate 5, 30 or 100 mg/kg once a day for 7 consecutive were days significantly decreased the blood glucose level from 228.3 ± 5.7; 228.3 ± 10.0 and 266.3 ± 34.3 mgldL to 151.0 ± 22.3; 140.7 ± 19.9 and 113.3 ± 26.7 mgldL, respectively (F(4 .• ) = 8.0004, P = 0.001). The repeateJ treatments of vanadyl sulphate could restore the diameter size of islet langerhans which was previously atrophied in diabetic state. At 5 mglkg dose, there was an initiation of bela pancreatic cell repairs, apparent activity of the nucleus cell, and a decrease in apoptosis. This condition was closer to nonnal statewith higller dose. Moreover, treatment with 100 mglkg showed proliferation of beta pancreatic cells. At hind paw muscles, the diameter size and glycogen had increased. At adipose tissue, vanadyl sulphate administration caused an increase of fatty drop in adipose cell and more homogenous cell without pseudopodia. Taken together, repeated treatments of vanadyl sulphate were effective to protect the morphological change ofbeta pancreatic cell, muscle and adipose tissue in the streptozotocin-induced diabetic mice

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