Gatot Soegiarto and Agustina Tri Endharti and Wibi Riawan and Anang Endaryanto and Subijanto Marto Sudarmo (2020) Lung Dendritic Cells Express Higher Stress Proteins on Higher Allergen Dose Exposure and Contribute to Allergen Tolerance Induction. Journal of International Dental and Medical Research, 13 (2). pp. 778-784. ISSN 1309100X

	Text (Penilaian Karil) Karil Artikel 3.pdf Download (628kB)
	Text (Artikel) Artikel 3.pdf Download (4MB)
	Text (Bukti Korespondensi) koresponden dendritic 1_merged.pdf Download (640kB)
	Text (Similarity) Turnitin Artikel 3.pdf Download (2MB)

Abstract

Different doses of allergen lead to different T lymphocyte responses which partly explain the phenomenon of low or high dose tolerance. Dendritic cells (DC) are responsible for driving the T lymphocyte responses to a variety of exogenous stimuli, but the mechanisms are not completely understood. Elucidate the mechanisms of how DCs drive the differentiation of T lymphocytes in response to different doses of allergen. Groups of male BALB/c mice (n=4-5) were sensitized intraperitoneally with sham or different doses (low: 10 g, high: 1000 g) of Der P1 (house dust mite allergen). They were exposed daily to aerosolized Der p1 allergen for 7 consecutive days. Different concentrations of Der p1 solution were nebulized: sham, low dose (15 g/mL), or high dose (1500 g/mL). Bronchoalveolar lavage fluid (BALF) was obtained from the lungs. Levels of IL-12 in BALF were measured. Lung tissue sections were then stained to detect the expression of Hsp70 by lung DCs. Lung DCs exposed to a higher sensitizing doses of Der p1 allergen tend to express significantly higher levels of Hsp70 and secrete higher levels of IL-12 in BALF. There was a significant positive correlation between the levels of Hsp70 expression by lung DCs with IL-12 levels in BALF (r=0.581). Higher exposure doses of allergen puts lung DCs under stressed condition thereby induced high expression of ‘stress proteins’ which may explain the mechanism of DCs drive the T lymphocyte response.

Actions (login required)

View Item