Adinda Nurinarasari Valentina NIM020710003, - (2010) Efektivitas Infusa Biji Pinang (Areca Catechu L.) I Dalam Menghambat Pertumbuhan Candida Albicans Pada Lempeng Akrilik (Analitik Eksperimental Laboratoris). Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background: Acrylic based denture are contaminated by Candida albicans that caused stomatitis while contact with palatal mucosa, especially Chronic Erythematous Candidiasis. Denture need to be cleaned by immersed it into chemical liquid In this case, the chemical liquid is areca seed infusion. This infusion contains alkaloid, tannin, flavonoid, and essensial oil that have antimicrobial effect which have antimicrobes activity especially against Candida albicans. Purpose: To find out the ability of areca seed infusion in inhibiting the growth of Candida albicans 's colony on acrylic based denture. Materials and Method: 24 acrylic plates that are used are heat cured acrylics (QC-20). During "dough stage" the acylic is embedded into the mold and the acrylic is then cured according to the manufacture's instruction. When the acrylic resin plates are done, it was being sterilized These acrylic were immersed in saliva for pellicle formation and immersed in Candida Albicans suspension. They were incubated at 3f1C for 24 hours. They were devided into 4 groups equally. Group 1 was immersed in sterile aquadest. Group 2 was immersed in 20% areca seed infusion. Group 3 was immersed in 30% areca seed infusion. Group 4 was immersed in 40% areca seed infusion. All groups being immersed for 30 minutes each. Saboroud's broth was used after the immersion so that the Candida albicans came off. Then, Saboroud's Dextrose Agar was used for growing Candida albicans. The colonies were counted using coloning forming unit (CFU) Result: The mean and standard deviation obtained for each group were: 1: -/ -, 2: 8500,000 / 4929,503, 3: 1666,667 / 1211,060, 4: 0,000 / 0,000. There is significant decrease of Candida albicans colonies growth between infusions with different concentration, since P(O.OOO) < a 0.05. Conclusion: Areca seed infusion does decrease the number of Candida albicans colonies growth. As high as the concentration, so that the ability of the infusion to kill the Candida albicans getting stonger.

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