Mimi Lamid and Ni Nyoman Tri Puspaningsih and WIDYA PARAMITA LOKAPIRNASARI (2009) Pemetaan Biodiversity Bahan Limbah Agroindustri Untuk Formula Pakan Komplit Menggunakan Enzim Lignoselulolitik Dalam Meningkatkan Ketahanan Pangan. Other thesis, UNIVERSITAS AIRLANGGA.

Text KKC LP 243-10 Lam-p.pdf Download (2MB)

Abstract

Potency produce waste of agroindustri high enough, but that way problems that happened is lowering of value of nutrition marked with obstetrical height of crude fibre cellulose, hemicellulose, lignine) and lower crude protein. Cellulose and hemicellulose available only some of small able to be exploited is most castaway become waste. Cellulose molecule and of hernicelulosa represent polysaccharide with tying 13- 1-4 glicosidic which is difficult to be digested by bacterium of rumen livestock of ruminantia, so that waste digesting of agroindustry become to lower. Usage of enzymes lignocellulolytic will be able to improve value of nutrition. Enzyme of cellulase consist of three enzyme component there are: endoglucanase ( endo-I,4-J3- D-Gluconase, or 1,4-D- Glucan 4-glucanohydrolase), eksoglucanase ( exo-I,4-J3-D-Glucanase, or 1,4- - Cellobiohydrolase D-glucan) and cellobiase ( - or glucosidase - Glucohidrolase Dglucosedi), while enzyme of xylanase consist of five enzyme component there are endoJ3- 1,4-xylanase, J3-xylosidase, - L-Arabinofuranosidase, - D-Glukuronidase, and acetyl xylan esterase. The aim of this research were exploration lignocellulolytic enzymes (cellulase and xylanase) from bacterium of rumen beef cattle, characterization optimum pH and temperature, determine molecular weight of protein cellulose and xylanase enzymes, and purification level of enzymes by diafiltrate and ion exchange chromatography. In this research, identification with Carboxyl Methil Cellulose (CMC) and Oat Splet Xylon has been done. The result showed that isolate BR2 have activity of endo-l,4-J3-D-Gluconase 0,28 Ulm and endo-B-I,4-xylanase 22,63 U/rnl. The optimum pH and temperature of celulase and xylanse were 6, 7 , 45°C, 50 °C. This process was started by producing cellulase and xylanase enzymes from isolate BR2, then precipitated it using ammonium sulphate with optimum concentration of saturated 40% and 60%, and the molecular weight protein of cellulase and xylanase enzymes were 49 kDa and 80 kDa. Based on experiment that celluloliytic enzymes from BR2 could hydrolyzed several specific substrate (CMC, pNPG dan pNPC), with activities crude extract cellulolytic are 3,51 UII, 1,86 UII dan 2,5 UII. Based on experiment that xylanolitic enzymes from BR2 could hydrolyzed several specific substrate (Oat spelt xi/an, pNP-X, pNP-G, pNP-acetyl acid, v pNP-A ), with activities crude extract xylanolitic were 0,433 U/ml, 0,034 U/m!, 0,018 U/ml, 0,008 U/ml, 0,0 II Ulm!. The best result of this diafiltrate on pNPG and pNPacetyl acid had level 8,01 and 49,90 from crude exctract. Beauchemin, K.A., D. Colombatto, D.P. Morgavi and W.Z. Yang. 2003.

Actions (login required)

View Item