A. A. M. N. Kasman and B. Santoso, - and Epy Muhammad Luqman, - and Widjiati, - (2021) Effects of Addition of Gonadotropin in the Media of Oocyte Maturation on the Embryo Cleavage. Journal of International Dental and Medical Research, 14 (1). pp. 412-418. ISSN 1309-100X

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Abstract

This research was performed to find the effects of addition of gonadotropin hormone in oocyte maturation in vitro on embryo cleavage. Gonadotropin hormone such as hMG 0,75 µg/ml and PMSG 15 IU were used in each oocyte maturation in vitro. Maturation media in this research was divided into 4 treatments such as Tc0, Tc+, Tc+hMG, and Tc+PMSG. Each treatment obtained 6 replicates. Oocyte maturation was carried out in each 100 µl TC 199 incubated for 24 hours. Staining of oocyte mature with aceto orcein and in vitro insemination were carried out in the middle of rosset. The research result showed that the average percentage of mature oocyte after maturation was 16.67% in the Tc0, 30% in the Tc+, 80% in the Tc+hMG, and 83.33% in the Tc+PMSG. Percentage of cleavage embryo was 5% for Tc0, 11.67% for Tc+, 43.33% for Tc+hMG, and 46.67% for Tc+PMSG. The result of correlation test on mature oocyte and cleavage was 0.932, the result of ANOVA test showed a significant difference, and the result of LSD test showed that there was difference between treatment groups of Tc0 and Tc+ compared to treatment group of Tc+hMG and Tc+PMSG, in both maturation and cleavage. This research concluded that the addition of gonadotropin obtained from hMg and PMSG into oocyte maturation media affected the increase in mature oocyte percentage and cleavage, there was no different result between the addition of hMG and PMSG on the percentage of mature oocyte and cleavage, and there was a correlation of mature oocyte on cleavage

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