Indentification of Caspase 3 Intensity in Kacang Goat Oocyte Post Vitrification using Confocal Laser Scanning Microscopy

Widjiati, - and Zakiyatul Faizah, - and Ninik Darsini, - and Viski Fitri Hendrawan, - and Helly Nurul Karima, - and Choirunil Chotimah, - and Sutiman Bambang Sumitro, - and Epy Muhammad Luqman, - (2020) Indentification of Caspase 3 Intensity in Kacang Goat Oocyte Post Vitrification using Confocal Laser Scanning Microscopy. Advances in Animal and Veterinary Sciences, 8 (12). pp. 1362-1366. ISSN 2309-3331, ISSN Online 2307-8316

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The research was aimed to find out caspase 3 intensity in Kacang goat oocyte post vitrification. The high caspase intensity will affect the viability of post thawing oocytes so that it will affect the oocyte quality and fertilization rate. The intensity of caspase 3 expression occurs because of the bond between caspase 3 with the Flou 3 probe so it will emit a fluorescent color, which is captured by the CLSM microscope device. The benefits of this research were to measure the intensity of caspase 3 quantitatively so as to determine the oocyte quality post thawing and determine the quality of frozen oocytes as a source of gametes for fertilization purposes. There were 2 groups in the research; Kacang goat fresh oocyte and kacang goat oocyte that was vitrified (each group consisted of 5 replications). The procedure of this study including oocyte collection, oocyte vitrification, frozen oocyte warming and Caspase 3 intensity examination were labeled with anti rabbit Fluorescein Isothiocyanate (FITC) secondary antibodies and measurement with Confocal Laser Scanning Microscopy (CLSM). Caspase 3 intensity of both groups was observed using CLSM. The results showed that caspase 3 intensity in the oocyte group which had been frozen by vitrification was higher significant than fresh oocytes (P < 0.05). The research concluded that caspase 3 intensity of frozen oocyte post vitrification was higher than that of fresh oocyte

Item Type: Article
Uncontrolled Keywords: Oocyte, Kacang goat, Vitrification, Caspase 3 intensity, Confocal Laser Scanning Microscopy
Subjects: S Agriculture > SF Animal culture > SF380-388 Goats
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF910 Other diseases and conditions
Divisions: 06. Fakultas Kedokteran Hewan > Anatomi Veteriner
Widjiati, -NIDN0015046204
Zakiyatul Faizah, -NIDN0026107708
Ninik Darsini, --
Viski Fitri Hendrawan, --
Helly Nurul Karima, --
Choirunil Chotimah, --
Sutiman Bambang Sumitro, --
Epy Muhammad Luqman, -NIDN0013126703
Depositing User: Nn Erna Dwi Indriyani
Date Deposited: 11 Apr 2023 01:41
Last Modified: 11 Apr 2023 01:41
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