Calcium Ionophore Supplementation in In vitro Fertilization Medium towards Fertilization Rates of Bean Goats (Capra hircus)

Azizah Yasita Najiati, - and Tjuk Imam Restiadi, - and Sri Pantja Madyawati, - and Imam Mustofa, - and Maslichah Mafruchati, - and Widjiati, - and Viski Fitri Hendrawan, - and Epy Muhammad Luqman, - (2022) Calcium Ionophore Supplementation in In vitro Fertilization Medium towards Fertilization Rates of Bean Goats (Capra hircus). Ecology, Environment and Conservation, 28 (2). pp. 635-641. ISSN 0971–765X

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In vitro fertilization is assisted reproduction technology that can overcome infertility problems. This method can produce embryos with high quality in large quantities. In its application, the success rate of IVF is still relatively low. This is due to the poor quality of oocytes and spermatozoa, which can cause IVF failure. The absence of oocyte activation during fertilization also results in failure of fertilization. Calcium ionophore is known to be able to increase the oocyte activation process. This study aims to prove that Calcium ionophore can increase the rate of fertilization in goat oocytes in vitro. This study was divided into two groups: the control group was fertilized oocytes using a medium without Calcium ionophore supplementation, the treatment group was fertilized oocytes using a medium supplemented with Calcium ionophore at a dose of 5.2 µl/ml. Fertilization rates are observed based on the formation of zygote or two pronuclear (2PN) in the cytoplasm of oocytes and observed under an inverted microscope. The data obtained were analyzed using the T test. The results of the study after being analyzed statistically showed that there were significant differences between the control group and the treatment group (P<0.05). Fertilization rates in the treatment group (93.12±13.7) showed higher results than the control group (65.96±28.36). Based on these results it can be concluded that calcium ionophore supplementation at a dose of 5.2 µl/ml in the fertilization medium can increase oocyte activation thereby increasing the rate of oocyte fertilization of the goat nuts

Item Type: Article
Uncontrolled Keywords: Food production, Infertility, Oocyte activation, Medium, Supplementation
Subjects: S Agriculture > SF Animal culture > SF380-388 Goats
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF811-909 Veterinary medicine of special organs, regions, and systems
Divisions: 06. Fakultas Kedokteran Hewan > Anatomi Veteriner
Azizah Yasita Najiati, --
Tjuk Imam Restiadi, -NIDN0009116110
Sri Pantja Madyawati, -NIDN0010026308
Imam Mustofa, -NIDN0027046003
Maslichah Mafruchati, -NIDN0010046205
Widjiati, -NIDN0015046204
Viski Fitri Hendrawan, --
Epy Muhammad Luqman, -NIDN0013126703
Depositing User: Nn Erna Dwi Indriyani
Date Deposited: 11 Apr 2023 02:50
Last Modified: 11 Apr 2023 02:50
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