Hawin Nurdiana, - (2007) Efek Pemberian Deksametason Dosis Rendah Dan Tinggi Terhadap Jumlah Retikulosit, Eritrosit Dan Kadar Hemoglobin Pada Tikus Putih Jantan (Rattus Norvegicus Galur Wistar): Penelitian Eksperimental Laboratoris. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The effect of low and high dose dexamethasone administration on reticulocyte, erythrocyte count, and haemoglobin (Hb) level remains controversial. This study is aimed to examine the effect of low and high dose dexamethasone on rats. 28 rats are chosen and grouped into control group and treatment group. Separate pretest received no treatment, posttest control groups received normal saline intramuscular injection and treatment groups received dexamethasone intramuscular injection, 0.1 mg I 200 g BW for low dose and I mg I 200 g BW for high dose, injection volume was 0.1 ml.The analysis unit was blood taken directly from heart at 24 hours after 6 days with I injection/day, and subjected to reticulocyte examination (%), erythrocyte (1061111), and haemoglobin (gldl). Results obtained in separate pretest was reticulocyte 1.7143 ± 1.0319 %; erythrocyte 8.1686 ± 0.4008 1061111; and Hb 15.0429 ± 0.8364 gldl. Group with low dose dexamethasone injection had reticulocyte 3.0714 ± 0.6317 %; erythrocyte 8.7129 ± 0.3398 1061111; and Hb 15.4286 ± 0.7319 gldl. Group with high dose dexamethasone injection had reticulocyte 1.7429 ± 0.4036 %; erythrocyte 8.6357 ± 0.4660 1061111; and Hb 15.1429 ± 1.3352 gldl. And the posttest control was reticulocyte 2.7857 ± 1.0961%; erythrocyte 8.2500 ± 0.5426 1061111; and Hb 13.8571 ± 0.9589 gldl. The results of Manova test between the posttest control and separate pretest show significant difference ( Hotelling's trace, p = 0.001), was maturation effect. Data were analyzed hereinafter using data from group with low and high dose dexamethasone injection that accurated with delta posttest control and separate pretest. The results of Manova test between the separate pretest and low dose dexamethasone injection show significant difference (Hotelling's trace, p = 0.025). While univariat test the difference was found in erythrocyte (p = 0.038 ) and Hb (p = 0.003) . The results of Manova test between the separate pretest and high dose dexamethasone injection didn't show difference (p = 0 .153) , but univariat test revealed difference in reticulocyte (p = 0.028). In conclucion, low dose dexamethasone administration may increase erythrocyte and Hb, while high dose dexamethasone administration may decrease reticulocyte.

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