Mohamad Fadjar, - (2011) Kloning Dan Ekspresi Gen Lactonase Dari Pseudomonas Aeruginosa Pa01, Sebagai Kandidat Inhibitor Autoinducer Quorum Sensing. Disertasi thesis, Universitas Airlangga.

	Text DIS K 68 02 ROE D.pdf Download (31MB)
	Text DIS M 01 12 FD K.pdf Download (46MB)

Abstract

Lactonase is an enzyme produced by several bacteria species which targeted and activated acylated homoserine lactones (AHL). AHL-Lactonase will hydrolyze the homoserine Lactone ring from AHL signal which effected to bacterial communicatlon and density increasing can not be happened, otherwise the bacterial virulence can be protected. In order to serve the demand of Lactonase, Cloning of Lactonase encoding gene from P. aeruginosa PA01 was done and transformed to E. coli M15 [pREP4]. The exact Lactonase gene cloning was proved using restriction enzyme test of Sal/ and Pstl. Conclusions of this research are :1) The long strand of the DNA of AHL-Lactonase encoding gene from P. aeruginosa PA01 was 783 bp, it is a ha/S gene, 2). The exact cloning of Lactonase gene in plasmid construction with Lactonase encoding gene in E. coli M15[pREP4] host cell was prove through Sal/ and Pstl restriction enzyme test, 3) Recombinant Lactonase protein was a putative Lactonase. It has a molecule protein mass of 30 kDa measured through SDS-PAGE and Western blot, 5) Activity test of recombinant Lactonase protein using N-hexanoyi-L- homoserine lactone (3 oxo-C6- HSL) substrate produced a product, while the exact compound was not known yet. While suggestions for this research are : 1. Need to do another Lactonase research which come from local bacteria species, 2. Need to make activity test for recombinant Lactonase protein as well to another suitable AHL-Lactonase substrates. 3 Need to do a direct activity test for this recombinant Lactonase protein to pathogenic bacteria in aquaculture.

Actions (login required)

View Item