Imam Suryanto, - (1999) Komparasi Teknik Minipreparasi DNA Plasmid Escherichia Coli Antara Metode Modifikasi Lisis Alkali Dan Metode Pemanasan: Penelitian Eksperimental Laboratorik. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this study is to initialize the characterization of the DNA plasmid minipreparat ion method . We have addressed this problem to compare these both methods, the alkali lysis modificated method and the boiling method. Six different E. coli which contain of YCp50 plasmid where used in this laboratory experiment with a Post Test Only Control Group Design. Purified DNA plasmid was demonstrated in both of these two methods and were measured for purity level by UV spectroscopy. Determination of DNA fragment cleavaged by BamHl, EcoRl and EcoRV was shown by running in gel electrophoresis and exposed by UV-transiluminator . Result were given as qualitative values. Wilcoxon’s Rank Sum Test and unpaired T test were used for statistical evaluation of the data. The result show that the yield of DNA plasmid by Alkali lysis modificated method ishigher than boilling method with significancy (p<0,05).

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