Muhammad Ali Shodikin, - (2009) Penelaahan Aktditas Antimikroba Ekstrak Etanol Buah Dan Daun Mahkota Dewa (Phaleria Macrocarpa) Terhadap Bakteri Pseudomonas Aeruginsa Menggunakan Cara Dilusi Agar Dan Mikroskopi Elektron. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Pseudomonas aeruginosa can develop some resistance patterns to some antimicrobial agents. The new potent antimicrobials not only expensive but also own some side effects. The research must be conducted to found new antimicrobial substance from natural product which cheap, effective and safe. Phytochemical screening of Phaleria macrocarpa fruits and leaves shown the existence of flavonoid, polyphenol, saponin, tannin compounds that known had antimicrobial activity. The aims of this experimental laboratory research were determine the antimicrobial activity, the minimum inhibitory concentration (MIC), the nature of antimicrobial activity, and also the antimicrobial potency comparison of ethanol extract of Phaleria macrocarpa fruits and leaves against P.aeruginosa. Three steps research were conducted: (1). Ethanol extraction of Phaleria macrocarpa fruits and leaves by maceration, (2). Agar dilution antimicrobial testing against P.aerufinosa ATCC 27853. The Muller Hinton Agar contain extract by concentration: 5xlO ~g/ml, 4xl04 ~g/ml, 3x104 ~g/ml, 2x104 ~g/ml, Ix104 ~g/ml, and 0 ~g/m, (3). Scanning Electron Microscopy (SEM) observation. The condensed ethanol extract was resulted from extraction of Phaleria macrocarpa fruits and leaves. In this work, we found the MIC of ethanol extract of Phaleria macrocarpa fruits and the MIC of ethanol extract of Phaleria macrocarpa leaves against P.aeruginosa were> 5x104 ~g/ml and 4xl04 ~ ~g/ml, respectively. SEM observation demonstrated the antimicrobial activity of ethanol extract of Phaleria macrocarpa leaves is bacteriostatic at 4x104 ~g/ml but bactericide at 8xl04 ~g/ml . The conclusion of this research is ethanol extract of Phaleria macrocarpa leaves has stronger antimicrobial activity against P.aeruginosa than ethanol extract of Phaleria macrocarpa fruits. However, ethanol extract of Phaleria macrocarpa leaves had low antimicrobial activity against P.aeruginosa, so that improper to use it as therapeutic agent against P.aeruginosa.

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