Analisis Genotipe, Subgenotipe dan Subtipe Virus Hepatitis B (VHB) Pada Genom Regio S (Surface) Dari Pasien Dengan HBsAg Positif di Kecamatan Mengwi, Kabupaten Bandung, Bali

Made Agus Hendrayana, - (2010) Analisis Genotipe, Subgenotipe dan Subtipe Virus Hepatitis B (VHB) Pada Genom Regio S (Surface) Dari Pasien Dengan HBsAg Positif di Kecamatan Mengwi, Kabupaten Bandung, Bali. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Indonesia has a high Endemic level of hepatitis B infection. Since the last decade, Mengwi has become a developed region with the rapid growth of social economic, demographic and dynamic inhabitants. This condition can contribute the varieties of HBV genotype, subgenotype, and subtype. The purpose of this research was to analyze the HBV genotype, subgenotype, and subtype on patients who possessed hepatitis signs and symptoms and came to Puskesmas Mengwi I. Badung, Bali. All serum samples were examined for ALT/SGPT level and detection for HBsAg by using enzyme-linked immunosorbent assay (ELISA) method. Serum of HBsAg Positive samples were used to identify the HBV genotype, subgenotype, and subtype. We carried out DNA extraction from serum of HBsAg Positive samples. HBV subtype were determined by using the analysis of amino acid substitutions at positions 122, 127, 134, 159, 160, and 117 ofS gene. This study used 75 serum samples taken from patients, two patients (2,7%) were found with high level of ALT. HBsAg Positive was found in 11 from 75 serum samples (14,7%). HBV genotype B was found predominantly, ten of eleven isolates (90,9%) belonged to genotype B and the other one (9,1%) to genotype C. Seven of eleven examined isolates belonged to subgenotype B3, three to subgenotype B7 and two were not classified to any of subgenotype yet because they did not fit in any branch of subgenotype B on the philogenetic tree. One of eleven examined isolates belonged to subgenotype C 1. Subtype adw2 was found on ten of eleven isolates (90,9%), followed by subtype adrq+ was found only on one sample (9,1%). Interestingly, in this study we found all of genotypes B were subtype adw2, and genotype C sample was subtype adrq+

Item Type: Thesis (Thesis)
Additional Information: KKA KK TK T 02/11 Hen a
Uncontrolled Keywords: Genotypes, subgenotypes, subtypes, hepatitis B virus, Mengwi
Subjects: R Medicine > RC Internal medicine > RC31-1245 Internal medicine
Divisions: 01. Fakultas Kedokteran > Ilmu Kedokteran Tropis
Creators:
CreatorsNIM
Made Agus Hendrayana, -NIM090810200 M
Contributors:
ContributionNameNIDN / NIDK
Thesis advisorRetno Handajani, -NIDN-
Thesis advisorMaria Inge Lusida, -NIDN-
Depositing User: sugiati
Date Deposited: 28 Mar 2023 03:54
Last Modified: 28 Mar 2023 03:54
URI: http://repository.unair.ac.id/id/eprint/121040
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