Maria Paulina Budyandini Dyah Pramesti (2003) Aplikasi Teknik Freeze-Drying Untuk Preservasi Sperma Manusia : Penelitian Eksperimental Laboratoris. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The objective of this study was to evaluate the application of treezedrying technique on human sperm preservation and to determine the influence of freezedrying process and 7 days storage under 4°C temperature on human spenn DNA integrity. The Posttest Only Control Group Design was used in this study. Population was sperm of adult male over 20 years old. The spenn should be in nonnozoospennia category and was obtained with consideration on WHO methode. The sample size was 12, obtained with simple random sampling methode. Comet assay was used to determine DNA integrity before freezedrying, immediately after freezedrying and 7 days storage after freezedrying. Manova was used to test the hypothesis. The results were : no significant difference in DNA integrity between fresh group and freeze-dried group (p>O,OS); there was very significant difference in DNA integrity between fresh group or freezedried group compared with freeze-dried + 7 days storage under 4°C temperature (p<O,Ol).

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