Gondo Mastutik, Gondo and Rahmi Alia, Rahmi and Alphania Rahniayu, Alphania and Anny Setijo Rahaju, Anny and Nila Kurniasari, Nila and Suhartono Taat Putra, Suhartono Genotyping Of Human Pappilomavirus In Cervical Precancerous Lesion And Squamous Cell Carcinoma At Dr. Soetomo Hospital, Surabaya, Indonesia. African Journal of Infectious Diseases, 12 (1). ISSN 2006-0165

	Text (Artikel) 1 ARTIKEL.pdf Download (1MB)
	Text (Laik Etik) 1 etik.pdf Download (271kB)
	Text (Kualitas Karil & Kesesuaian Bidang Ilmu) Karil (1)-1.pdf Download (99kB)
	Text (Turnitin) 1 turnitin.pdf Download (1MB)
	Text (Kualitas Karil & Kesesuaian Bidang Ilmu) 1 Karil.pdf Download (396kB)

Abstract

Abstract Background: Cervical cancer caused by human papilloma virus (HPV), is the second most common cancer for women. This cancer is distributed worldwide, with ~80% of cases are found in the developing countries. In Indonesia, data of HPV genotypes are still limited and do not represent all regions of the country. Thus, here we report genotyping of HPV samples collected from the Dr. Soetomo Hospital Surabaya Indonesia patients, in 2013. Materials and method: A cross sectional study was performed using 68 paraffin blocks of low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), and squamous cell carcinoma (SCC) cervix. Result: This study showed that HPV genotypes found in LSIL samples are HPV 16, 18, 6/33 or 68/72. Furthermore, those in HSIL are HPV 16, 18, 52, 59, 67, 6/18, 6/45, 16/67, 26/61, or 52/67, while in SCC are HPV 16, 18, 45, 52, 56, 16/18 or 16/45. Single-genotype infection, i.e. by HPV 16, 18, 45, 52, 56, 59, or 67, was observed in 86.77% (59/68) of samples, whereas multiple-genotype infections, i.e. by HPV 6/18, 6/33, 6/45, 16/18, 16/45, 16/67, 26/61, 52/67, or 68/72, was found in 13.23% (9/68) of the samples. Conclutions: The mostly HPV genotype identified in this study is HPV 16 (62.68%), then followed by HPV 18 (20.9%), HPV 45 (5.97%), 52 (5.97%), and 67 (4.48%). HPV 16 and 18 have used as vaccine, and HPV 45 has cross reaction with HPV 18, then HPV 52 and 67 should be considered as the second-generation HPV vaccines. Keywords: Human Papillomavirus; high-grade squamous intraepithelial lesion; low-grade squamous intraepithelial lesion; squamous cell carcinoma.

Actions (login required)

View Item