Fadila Zikri Amanda, - and Sri Pantja Madyawati, - and Isnaini Fadilah, - and Erma Safitri, - (2019) Antisperm Antibody in Repeat Breeder Friesian Holstein Cows at KPSP Setia Kawan Nongkojajar, Tutur District, Pasuruan, Indonesia. Indian Veterinary Journal, 96 (10). pp. 20-23. ISSN 0019-6479 e-ISSN 0974-9365

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Abstract

This study presents a laboratory explorative method by using sample of cervical mucus and blood serum from 11 Holstein Friesian (HF) cows, aged 4 to 9 years age, normal estrus cycle and gave birth before. They were divided into 2 groups, 10 dairy cows that have history of repeat breeders (4 times or more AI) (RB) and 1 normal breeder (2 times AI) as a control (C). Samples were examined using indirect-ELISA test method. The results of this study indicate that the antisperm antibody concen tration that appears in cervical mucus samples and blood sera of control group has a lower concentration value (0.342 and 54.860 ng/mL) than RB group (233,776 and 944,531 ng/ml).

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