Erma Safitri, - and Pudji Srianto, - and Thomas Valentinus Widiyatno, - and Willy Sandhika, - and R. Heru Prasetyo, - (2018) Viability of Rabbit Adipocyte Stem Cells Cultured Under Different Oxygen Concentrations In Vitro. The Philippine Journal of Veterinary Medicine, 55. pp. 1-8. ISSN 0031-7705

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Abstract

The aim of this research was to determine if rabbit adipocyte stem cells (r-ASCs) can still be viable before transplantation when these are cultured in vitro under hypoxic preconditioning (3% O2 concentration). Samples were divided into two groups, one under hypoxic preconditioning and the other in hyperoxia (20% O2 concentration). Observations were made through flow cytometry, immunofluorescence and immunocytochemical analyses. Flow cytometric analysis showed that in r-ASCs hypoxic culture, the levels of CD90+, CD44+ and CD45- were unaltered, but changed under hyperoxic culture, indicated by the down-regulation of CD90+ and CD44+, and up-regulation of CD45-. Meanwhile, immunocytochemical and immunofluorescence analysis showed that under hypoxic preconditioning, r-ASCs culture expressed quiescent cells with p63 as marker, but the latter remained unexpressed in hyperoxic culture. In conclusion, hypoxic preconditioning with 3% O2 concentration supported r-ASCs in sustaining viability before transplantation in rabbit.

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