Sri Wahjuni (1999) Pengaruh Substitusi Berbagai Kadar Minyak Ikan Lemuru dalam Diit terhadap Profil Lipid Serum serta Pengaruhnya terhadap Peroksidasi Lipid tanpa Maupun dengan Suplementasi vitamin E pada Tikus. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of the current study was to determine the effects of substituting variuous concentrations of lemuru fish (Sardtnetia Longtceps) oil (LFO) in the rafs diet on the serum triglyceride (TG), total cholesterol (TC), LDL-cholesterol (LDL-C) and HDLcholesterol (HDL-C). The effects of LFO on serum malondialdehyde (MDA) in the presence or absence of dietary vitamin E(vitamin E) was also assessed. For this pmpose male Wistar rats (Rattus norvegicus), 4 weeks of age, weighing 70-72 g were used. The rats were first fed a standard diet and tap water from the municipal supply for 1 month, followed by a high fat diet for 6 weeks. The rats were then divided into 9 groups each fed a different diet as follows: A1 = LFO (-), vitamin E (-) (control group) A2 = LFO (-), vitamin E 6 mg/Kg A3 = LFO (-), vitamin E 8 mg/Kg B1 = LFO 10%, vitamin E (-) B2 = LFO 100/o. vitamin E 6 mg/Kg BJ = LFO 1 00/o, vitamin E 8 mg/Kg C1 = LFO 15%, vitamin E (-) C2 = LFO 15%, vitamin E 6 mg/Kg C3 = LFO 15%, vitamin E 8 mg!Kg Serum lipid profile (TG, TC, LDL-C, and HDL-C) were determined spectrohotometrically (). = 500 nm) using Boehringer-Mannheim Kit (TG: GPO-PAP~ TC, LDL-C, and IIDL-C: CHOD-PAP), while MDA were assesed using the method ofEpinosa and Mansila The obtained data were then analyzed statiscally at 5% confidence level, using oneway anova for lipid profile and two-way anova for MDA followed by LSD test as necessary. A significant increase in body weight were obsetved in all rats belonging to all 8 experimental groups as compared to control (A1 group). however, no further conclusion on the effect of LFO and vitamin E could be drawn because no consistent trend could be detected The effect on lipid profile was assessed by comparison between groups Al, Bl and C1 (those groups without addition of vitamin E). LFO decreased senun TG, TC, and LDL-C. 10% LFO decreased TG and TC significantly, but increasing LFO from 10% to 15% did not result in a further significant decrease. On the other hand, 100/o LFO did not significantly decrease LDL-C : •" significant decrease was only found at 15% LFO. While LFO decreased TG, TC, and LDL-C, LFO increased HDL-C. Similar • to LDL-C, 10% LFO did not significantly increase HDL-C. The significant increase was only found at 15% LFO. The effect ofLFO and vitamin Eon serum MDA was assessed on all 9 groups (Al through CJ). LFO and vitamin E. either singly or combined, decreased serum MDA, 10% LFO decreased MD A. significantly, and a further significant decrease oc<:ured if LFO was increased from 10% to 15%. This relation held true, irrespective of whether or not vitamin E was present. Six mg vitamin E decreased serum MDA significantly, but no further significant decrease of serum MDA occurcd if vitamin was increased from 6 mg to 8 mg. Again this relation held true irrespective of the presence or absence of LFO. It can thus be concluded that LFO and vitamin E reinforced each other in their effects on serum MDA.

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