Supriyadi
(2004)
Efek Radiasi Ionisasi Dosis Tunggal terhadap Apoptosis Sel Fibroblas Jaringan Pulpa: Penelitian eksperimental laboratorlam pada tikus putih (Ratus norvegicus).
Thesis thesis, UNIVERSITAS AIRLANGGA.
Abstract
OBJECTIVE. The purpose of this study was to prove the apoptotic increasing of
fibroblas pulp cell caused by single dosage ionizing radiation: in vivo.
MATERIAL AND METHODE. This study is laboratory experimental research
that use the Post-Test Control Group Design. The sample research was rats, wistar
strain, male, 3-4 month of age, 150- 200g of weight and healthy. There were 24
rats that were divided into 4 groups of 6 rats: the first group was used as a control
group (no radiation or 0 rad radiation); the second, the third and the forth group
were consecutively induced by I 00, 200 and 400 rad single dosage radiation. The
source of radiation was Cobalt 60 that was exposured to the head of each rat. The
entire sample was hereinafter sacrificed 24 hours after radiation exposure, and the
lower insicivus were taken for histopatology specimen processed . The detection
of apoptotic using the TUNEL Assay method, so the specimens were stained with
histochemical technique using 57101 Apoptag Plus Peroxidase In Situ Apoptosis
Detection Kit. The apoptotic of fibroblast pulp cells were counted under light
microscope in the magnification of 400x, by three competence observers with
blind test technique. Observation and counting of apoptotic cells (percentage
mat hod) were conducted at two sides (labia.! and palatal side of the teeth) below
odontogenic and free-cell zone, then the mean value of the result of the counting
were taken. The data were statistically analyzed using one-way ANOV A and LSD
test at sit,mificant level (a) of0.05.
RESULTS. The results of this study indicated that there were significant
percentage increasing the apoptotic of fibroblast pulp cell followed by increasing
the radiation dosage of 0, I 00 and 200 rad, but the percentage was then decreasing
significantly at 400 rad radiation dosage. The one-way ANOV A test proved that
there was a significant difference the apoptotic percentage of fibroblast pulp cell
among the four groups (p < 0.05 ).
CONCLUSION. The conclusion of this study was the percentage increasing the
apoptotic of fibroblast pulp cell was followed by the increasing of radiation
dosage 0, 100 and 200 rad, but the decreasing of these was occurred after using
400 rad radiation dosage. The apoptotic decreasing at 400 rad radiation dosage
was supposed to caused the increasing of another cell death form, that is necrosis
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