Dwi Rahmawati, - and Alexander Patera Nugraha, - and Alivianda Zahrina Saraya, - and Nastiti Faradilla Ramadhani, - and Wibi Riawan, - and Igo Syaiful Ihsan, - and Diah Savitri Ernawati, - and Ida Bagus Narmada, - and Tania Saskianti, - and Fianza Rezkita, - and Andari Sarasati, - and Albertus Putera Nugraha, - and Tengku Natasha Eleena Binti, - and Tengku Ahmad Noor, - (2023) Basic Fibroblast Growth Factor Expression after Gingival Mesenchymal Stem Cell’s Metabolite Provision in Lipopolysaccharide induce inflammatory bone resorption in vivo. Journal of International Dental and Medical, 15 (2). ISSN 1309-100X

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Abstract

Normal bone is experience bone remodeling all the time where bone resorption and bone formation are balanced. Some chronic disease, such us periodontitis could affect the bone remodelling causing bone resorption is higher than bone formation. Basic Fibroblast Growth Factors (bFGF) has role on osteogenesis which can help in increasing bone formation. Gingival Mesenchymal Stem Cells’s Metabolite (GM SCM) is medical wasted products from Mesenchymal Stem Cells (MSC) which has various function. Aim of this study is to investigate the metabolites of GM SC effect on bFGF expression in inflammatory bone resorption caused by lipopolysaccharide. The 20 experimental animals were separated into four groups: control (C): 100 g PBS day 1-7, LPS group: 100 g LPS day 1-7, LPS+GM SCs' metabolite group: 100 g LPS + 100 g GM SCs' metabolite day 1-1-7, and GM SCs' metabolite group: 100 g M-GM SCs day 1-7. Escherichia Coli LPS was employed to trigger bone resorption on the calvaria of an animal model. The dose of GM SCs metabolite administered is 100 g once day through subcutaneous injection. Furthermore, on day 8, all samples were sacrificed by cervical dislocation. To count the number of bFGF positive expressions in osteoblast in the calvaria of animal models, bFGF monoclonal antibody and Diaminobenzidine (DAB) is added, resulting in a brown precipitate developing where the antibody has attached. The statistical analysis was performed to examine the significantly different between groups (p<0.05). The expression of bFGF was significantly decreased in LPS induced bone resorption group (LPS group), however, after GM SCs’ metabolite provision, bFGF expression was significantly elevated in GMSCs metabolite and LPS induced bone resorption (LPS+GM SCs’ metabolite group) with significantly different (p=0.0001; p<0.05). The positive expression of bFGF in osteoblast was elevated after GM SCs metabolite provision in LPS-induced calvaria bone resorption in wistar rats (R. novergicus) by means of immunohistochemistry examination.

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