Sri Winarni (2010) Pengaruh Fraksi Etanol 96% dan Isolat Biji Koro Benguk (Mucuna Pruriens L) Terhadap Kualitas Spermatozoa Mencit (Mus Musculus) Terpapar 2-Metoksietanol (Penelitian Eksperimental Laboratoris). Thesis thesis, UNIVERSITAS AIRLANGGA.
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1. COVER .pdf Download (2MB) |
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2. ABSTRACT .pdf Download (167kB) |
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4. BAB 1 PENDAHULUAN .pdf Download (820kB) |
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8. BAB 5 ANALISIS HASIL PENELITIAN .pdf Download (4MB) |
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10. BAB 7 PENUTUP .pdf Download (151kB) |
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11. DAFTAR PUSTAKA .pdf Download (1MB) |
Abstract
Infertility incidence is increasing in the last 40 years. Approximately 10¬15% of population are infertile. Based on previous study, active ingredient of Mucuna pruriens L. or koro benguk (Paptlionaceae), the L-dopa, may affect the quality of spermatozoa. The objective of this research was to study the effect of 96% ethanol fraction and M. pruriens seed isolate on spermatozoa quality of mice exposed to 2-Methoxyethanol. L-dopa in 96% ethanol fraction of Al. pruriens seed was 14.7%, while that in the isolate was 56.57%. This was an experimental study using complete randomized design. Subjects were BALB/C mice (Mus musculus), consisting of 11 groups. Five groups served as control, 3 groups received subcutaneos injection of 2-ME fraction as much as 100 mg/kg.bw/day for 12 days, followed with 96% ethanol fraction starting from 14 mgikg.bwiday, 28 mg/kg.bwiday, and 56 mg/kg.bwiday for 51 days, and 3 groups served as isolate treatment group that received subcutaneous injection of 2-ME of 100 mg/kg.bw.day for 12 days, followed with isolate starting from 1.4 mg/kg.bw/days, 2.8 mg/kg.bw/days, and 5.6 mg/kg.bwidays for 51 days. The final observation was conducted by counting spermatozoa count, motility speed, spermatozoa viability, and percentage of normal spermatozoa morphology. The result of this study showed that 96% ethanol fraction and the isolate of M. pruriens seeds are able to increase motility speed, the percentage of spermatozoa viability, and the percentage of normal spermatozoa morphology in mice exposed to 2-ME (p < 0,05).
| Item Type: | Thesis (Thesis) | |||||||||
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| Additional Information: | KKA KK TKR 01/11 Win p | |||||||||
| Uncontrolled Keywords: | Mucuna pruriens L. L-dopa, mouse spermatozoa. | |||||||||
| Subjects: | R Medicine > RG Gynecology and obstetrics > RG1-991 Gynecology and obstetrics > RG484-485 Urogynecology and obstetric urology. Urogynecologic surgery | |||||||||
| Divisions: | 01. Fakultas Kedokteran > Kesehatan Reproduksi | |||||||||
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| Depositing User: | Sulistiorini | |||||||||
| Date Deposited: | 11 Apr 2023 02:29 | |||||||||
| Last Modified: | 11 Apr 2023 02:29 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/122900 | |||||||||
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