Silda Damayanti, ,- and Rahayu Kusdarwati, ,- and Hari Suprapto, ,- (2019) Bacterial resistance of Escherichia coli against antibiotics in Clarias batrachus digestion. AACL Bioflux, 12 (6). pp. 2195-2201. ISSN 1844-9166

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Abstract

Catfish (Clarias batrachus) is a freshwater fish with a high demand and an affordable price in Indonesia. However, in aquaculture, farmers usually use antibiotics for treatments, prophylaxis, growth improvement and feed efficiency. The excessive use of antibiotics can lead to bacterial resistance, especially for Escherichia coli, and can increase the risk of treatment failure in infected fish. This study aims to determine the resistance of E. coli found in the catfish digestion system to several antibiotics used in aquaculture. This study used a descriptive method with samples of 25 g from the digestive organs of Clarias batrachus. This study was conducted at the Education Laboratory of the Faculty of Fisheries and Marine Science, Surabaya, Universitas Airlangga, Indonesia. The main parameter observed was the inhibition zone produced by antibiotics, in the form of a clear zone. The determinations were made by a sensitivity test. E. coli bacteria obtained from the digestive organs of the catfish were isolated and several types of tests were conducted, E. coli assay, E. coli assertion, biochemical and antibiotic resistance tests. The resistance pattern of E. coli presents a resistance of 100% to tetracycline and ampicillin and 77.78% to chloramphenicol. The highest level of E. coli bacteria resistance was to tetracycline and ampicillin. However, gentamicin has the lowest resistance value and can be concluded that gentamicin still presents inhibitory abilities against pathogenic E. coli bacteria in catfish.

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