Sri Wigati Mardi Mulyani, - and Eha Renwi Astuti, - and Nastiti Faradilla Ramadhani, - and Tengku Natasha Eleena Binti Tengku Ahmad Noor, - (2023) Stromal Derived Factor-1, C-X-C Motif Receptor-4 and Vascular Endothelial Growth Factor Expression after Induction of Garcinia mangostana. L Peel Extract in Bone Marrow Mesenchymal Stem Cells Culture. Journal of International Dental and Medical Research, 16 (2). pp. 135-136. ISSN 1309-100X

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Abstract

survival was unavoidable when stem cell being transplanted. Garcinia mangostana. L Peel may possess beneficial active compound to enhance homing factor of stem cells. C-X-C Motif Receptor-4 (CXCR4), Stromal Derived Factor-1 (SDF-1) and Vascular Endothelial Growth Factor (VEGF) play an important role in stimulating endogenous Bone Marrow Mesenchymal Stem Cells (BMMSCs) mobilization. The aim of this research to find out whether mangosteen (G. mangostana. L) peel extract can induce the expression of a CXCR4, SDF-1 and VEGF in BMMSCs culture. BMMSCs were taken and isolated from femur of male Wistar rats (Rattus novergicus). BMMSCs were cultured then added with mangosteen (Garcinia mangostana. L) peel extract. In this study, the culture of MSCs divided into 2 groups, group 1: added G. mangostana. L peel extract and group 2: without G. mangostana. L peel extract. The examination of SDF-1and CXCR4 expression by using enzyme linked immunosorbent assay (ELISA), meanwhile the VEGF expression by using Immunocytochemistry. Statistical analysis was test using Levene test for variance homogeneity and normality test with the Saphiro-Wilk test. If the data is normally distributed then a different test is performed using the t-test (p<0.05). There was a significant difference in the expression of SDF1-CXCR4, and VEGF (p<0.05) in BMMSCs cells culture between two groups (p<0.05). Conclusion: G. mangostana peel extract is able to induce the expression of a number of proteins, growth factors and chemokines, such as VEGF, CXCR4, and SDF-1.

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