Eric Priyo Prasetyo, - and Mefina Kuntjoro, NIDN. 0029097905 and Febriastuti Cahyani, - and Setyabudi Goenharto, - and Widya Saraswati, - and Devi Eka Juniarti, - and Nike Hendrijantini, - and Ninuk Hariyani, - and Alexander Patera Nugraha, - and Fedik Abdul Rantam, - (2021) Calcium Hydroxide Upregulates Interleukin-10 Expression in Time Dependent Exposure and Induces Osteogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cells. International Journal of Pharmaceutical Research, 13 (1).

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Abstract

Background: Human umbilical cord mesenchymal stem cells (HUCMSCs) as one of mesenchymal stem cells source, have a promising potential for regenerative dentistry. Calcium hydroxide, a widely administered medicament for endodontic therapy, is used for its numerous benefits. The presence of calcium hydroxide is expected to bring a positive outcome on HUCMSCs. Purpose: This study aims to evaluate the anti-inflammatory effect through Interleukin-10 expression and osteogenic differentiation of calcium hydroxide on HUCMSCs. Methods: This study is an experimental laboratory research with controlled group design. HUCMSCs were grown in Minimum Essential Medium (MEM) Alpha containing 0.1 µg/ml of calcium hydroxide. Observation of interleukin-10 expression were conducted in 24 hours, 72 hours, and 168 hours. Osteogenic differentiation was observed with Alizarin Red S staining. Obtained data were analyzed with analysis of variance (ANOVA). The level of significance was set at 0.05. Results: Calcium hydroxide increases Interleukin-10 expression of HUCMSCs (P 0.000). Exposure time of 24 hours, 72 hours, and 168 hours exhibit increased expression. Exposure of 168 hours displayed the highest increase, followed by 72 hours and 24 hours as the lowest. Calcium hydroxide also induce osteogenic differentiation of HUCMSCs. Conclusions: Calcium hydroxide increases the expression of Interleukin-10 in time dependent exposure and induces osteogenic differentiation of HUCMSCs. The longer the exposure of calcium hydroxide to HUCMSCs, the higher the expression of interleukin-10. These findings support the use of calcium hydroxide on HUCMSCs for pulpal and periapical tissue regeneration.

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